Expression of high-mobility group box-1 in eutopic/ectopic endometrium and correlations with inflammation-related factors in adenomyosis

Figures & data

Table 1. RT-PCR primers.

Name	Primer sequence (5′–3′)	Annealing temperature (°C)	Size of product (bp)
HMGB1	F: GTGGCTCACGCCTGTAATCC R: GGCACAATCTCGGCTCACTG	60	230
TLR4	F: CCGCTTTCACTTCCTCTCAC R: CATCCTGGCATCATCCTCAC	60	118
IL-6	F: AGCCACTCACCTCTTCAGAAC R: GCCTCTTTGCTGCTTTCACAC	60	182
IL-8	F: CAAGAGCCAGGAAGAAAC R: TGGTCCACTCTCAATCAC	60	227
β-actin	F: CAAGATCATTGCTCCTCCTG F: CAAGATCATTGCTCCTCCTG	60	90

Figure 1. Expression of IL-6/IL-8 (A), HMHB1 (B), and TLR4 (C) in the CE, EuE, and EE groups. **p < .01.

Figure 2. Expression of HMHB1 (D) and TLR4 (E) proteins in the CE, EuE, and EE groups. **p < .01.

Figure 3. Expression of IL-6/IL-8 and TLR4 proteins in the CE, EuE, and EE groups.

Figure 4. Correlation of mRNA HMGB1 expression with TLR4, IL-6, and IL-8 (A), TRL4 with IL-6 and IL-8 (B), and TLR4 with HMGB1 (C) in the EuE and EE groups.

Table 2. Correlation between HMGB1 expression and IL-6 and IL-8 in EuE and EE.

		HMGB1
EuE	IL-6	0.470*
	IL-8	0.574**
	TLR4	0.538*
EE	IL-6	0.976**
	IL-8	0.650**
	TLR4	0.916**

**A significant correlation at 0.01 (bilateral).

*A significant correlation at 0.05 (bilateral).