Down-regulation of the FTO gene in follicular fluid of infertile women with ovarian endometriosis

Figures & data

Table 1. Characteristics of the OE and control patients whose samples were used for ELISA Analyses.

Parameter	Control (n = 37)	OE (n = 37)	p Value
Age (years)	31.59 ± 0.44	31.65 ± 0.49	0.935
AMH (ng/ml)	4.06 ± 0.47	2.72 ± 0.46	0.009**
Basal FSH (mIU/ml)	7.00 ± 0.34	8.41 ± 0.48	0.020*
AFC	14.24 ± 0.80	8.22 ± 0.82	<0.0001***
BMI (kg/m^2)	22.36 ± 0.60	21.80 ± 0.48	0.469
Duration of infertility (years)	3.74 ± 0.32	3.70 ± 0.42	0.555
Endometrial thickness (mm)	10.86 ± 0.36	10.71 ± 0.40	0.969

Compared with controls, *p < .05, **p < .01, ***p < .0001.

Figure 1. The concentrations of protein in follicular fluid (FF) of control group (control) and ovarian endometriosis group (OE). The concentrations of FTO and METTL3 in FF of women with OE (n = 37) were lower than that of the control group (n = 37, *p <.05, (A,C). The concentrations of KIAA1429 in FF of women with OE (n = 37) were higher than that of the control group (n = 37, ***p <.0001, E).

Figure 2. Expression of FTO in granulosa cells (GCs) of women with ovarian endometriosis (OE). (A) FTO levels were significantly lower in women with OE (n = 21) compared with women in control group (n = 19, *p <.05). (B) The content of m⁶A in GCs of women with OE (n = 10) was higher than that of the control group (n = 10, p >.05).

Table 2. ART cycle Characteristics in OE and control patients.

Parameter	Control (n = 37)	OE (n = 37)	p Value
IVF	27	26	–
ICSI	10	11	–
Dosage of gonadotropin (IU)	2412 ± 110.1	3154 ± 199.4	0.005**
Number of oocytes retrieved	15.41 ± 1.24	10.14 ± 0.94	0.001**
Number of mature oocytes	13.00 ± 1.18	8.92 ± 0.86	0.007**
Number of fertilized oocytes	8.92 ± 0.99	6.94 ± 0.74	0.117
Fertilization rate (%)	70.36 (330/469)	75.76 (250/330)	0.092
Cleavage rate (%)	87.27 (280/330)	81.20 (203/250)	0.045*
Number of embryos available	7.06 ± 0.85	5.22 ± 0.69	0.099
Available embryo rate (%)	76.47 (247/323)	77.69 (188/242)	0.734
Number of top-quality embryo	3.71 ± 0.76	2.14 ± 0.37	0.164
Top-quality embryo rate (%)	40.25 (130/323)	31.82 (77/242)	0.040*
Number of embryos transferred	1.44 ± 0.14	1.61 ± 0.14	0.366
Implantation rate (%)	39.29 (11/28)	27.27 (9/33)	0.991
Clinical pregnancy rate (%)	56.25 (9/16)	38.10 (8/21)	0.272

Compared with controls, *p < .05, **p < .01, ***p < .0001.