Figures & data
Figure 1. Vaspin promotes the viability of luteal cells. (A) CCK-8 to observe the effect of different concentrations of Vaspin on the viability of granulosa lutein cells (n = 3); (B-C) Western blot to assess the effects of different concentrations of Vaspin on the expression levels of CyclinD1 and CyclinB1 in granulosa lutein cells (n = 3). **p < 0.01, vs. Control.
Figure 2. Effect of Vaspin on the apoptosis of luteal cells. (A) Anneixn-FITC/PI staining to observe the effects of different concentrations of Vaspin on the apoptosis of granulosa lutein cells (n = 3); (B-C) Western blot to detect the effects of different concentrations of Vaspin on the expression levels of Bax and Bcl2 in granulosa lutein cells (n = 3). **p < 0.01, vs. Control. Bcl-2, B-cell lymphoma 2; Bax, Bcl-2 associated X.
Figure 3. Vaspin stimulates the angiogenesis of luteal cells. (A) Angiogenesis experiment to observe the effect of different concentrations of Vaspin on the angiogenesis ability of granulosa lutein cells (n = 3); (B-C) Western blot to estimate the effects of different concentrations of Vaspin on the expression levels of VEGFA and FGF-2 in granulosa lutein cells (n = 3). **p < 0.01, vs. Control. VEGFA, vascular endothelial growth factor A; FGF-2, fibroblast growth factor-2. Red arrows indicate formed blood vessels.
Figure 4. Vaspin up-regulates the steroidogenesis in luteal cells. A-B, ELISA to observe the impact of different concentrations of Vaspin on the production of P4 and E2 in granulosa lutein cells (n = 3). **p < 0.01, vs. Control. P4, progesterone; E2, estradiol.
Figure 5. Vaspin activates the activity of MEK/MAPK signaling pathway. (A-B) Western blot to observe the effects of different concentrations of Vaspin on the expression of MEK/MAPK signaling pathway-related proteins (p-MEK1, MEK1, p-p38, p38) (n = 3). **p < 0.01, vs. Control.
Supplemental Material
Download MS Word (205.5 KB)Data availability statement
The data used to support the findings of this study are available from the corresponding author upon request.