Association of the CYP17 gene polymorphism with risk for uterine leiomyoma in Brazilian women

Abstract

Background. Uterine leiomyoma is the most common pelvic tumor in women of reproductive age. It is well established that endogenous sex hormones are involved in disease pathogenesis, and polymorphisms in genes encoding enzymes which act in the metabolism of steroid hormones, such as that for cytochrome P450c17α enzyme (CYP17), may therefore play a role in fibroid genesis. Variations in this gene have been thought to influence the susceptibility to hormone-related diseases. A single nucleotide polymorphism (T→C) [rs1042386] in promoter region of CYP17 may alter its transcription. The present study was conducted to investigate the association between this polymorphism and the presence of uterine leiomyoma in Brazilian women.

Methods. Genotyping of CYP17 was performed in 121 uterine fibroid patients and 120 unaffected women, using polymerase chain reaction and restriction fragment-length polymorphism analysis.

Results. No significant difference in the CYP17 genotype distribution was noted between cases and controls (p = 0.165).

Conclusion. These findings suggest that the CYP17 gene polymorphism studied is unlikely to be associated with risk for uterine leiomyoma in Brazilian women.

Keywords:

• Uterine leiomyoma
• uterine fibroids
• hormone metabolism
• CYP17
• polymorphism
• single nucleotide polymorphism