Advanced search
Publication Cover
Platelets Volume 29, 2018 - Issue 7
Submit an article Journal homepage
1,351
Views
14
CrossRef citations to date
0
Altmetric
Plenary Paper

Not all light transmission aggregation assays are created equal: qualitative differences between light transmission and 96-well plate aggregometry

Melissa V. ChanThe Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, London, UKCorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0003-2154-1386View further author information
ORCID Icon,
Philip D. LeadbeaterIntensive Care Unit, Western General Hospital, Edinburgh, UKView further author information
,
Steve P. WatsonInstitute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UKView further author information
&
Timothy D. WarnerThe Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, London, UKView further author information
Pages 686-689 | Received 02 Mar 2018, Accepted 09 Apr 2018, Published online: 01 May 2018

Figures & data

Figure 1. Final aggregation as measured by (A) light transmission aggregometry and (B) 96-well aggregometry in the presence of vehicle (white), aspirin (ASA, 30 μM; black), and AR-C66096 (AR-C, 1 μM; grey) in response to arachidonic acid (AA; 0.03–1 mM), ADP (0.005–40 μM, collagen (0.01–40 μg/ml), and U46619 (0.005–40 μM). Data were analyzed by one-way or two-way ANOVA and assessed as significant to *p < 0.05. N = 5 for each.

Figure 1. Final aggregation as measured by (A) light transmission aggregometry and (B) 96-well aggregometry in the presence of vehicle (white), aspirin (ASA, 30 μM; black), and AR-C66096 (AR-C, 1 μM; grey) in response to arachidonic acid (AA; 0.03–1 mM), ADP (0.005–40 μM, collagen (0.01–40 μg/ml), and U46619 (0.005–40 μM). Data were analyzed by one-way or two-way ANOVA and assessed as significant to *p < 0.05. N = 5 for each.

Figure 2. Final aggregation in light transmission aggregometry with stir speeds of 100, 300, and 1200 rpm in the presence of vehicle (white), aspirin (ASA, 30 μM; black), and AR-C66096 (AR-C, 1 μM; grey) in response to (A) ADP (3 μM), (B) collagen (3 μg/ml), and (C) U46619 (3 μM). Data were analyzed by one-way ANOVA and assessed as significant to p < 0.05; * vs 100 rpm and $ vs vehicle. N = 5 for each.

Figure 2. Final aggregation in light transmission aggregometry with stir speeds of 100, 300, and 1200 rpm in the presence of vehicle (white), aspirin (ASA, 30 μM; black), and AR-C66096 (AR-C, 1 μM; grey) in response to (A) ADP (3 μM), (B) collagen (3 μg/ml), and (C) U46619 (3 μM). Data were analyzed by one-way ANOVA and assessed as significant to p < 0.05; * vs 100 rpm and $ vs vehicle. N = 5 for each.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.