Comparative study on the stability of soybean (Glycine max) β-conglycinin in vivo

Figures & data

Table 1. Composition of the diets, as-fed basis (%).

Item	3–5^a	5–10^a	Group A^b	10–20^a	20–60^a	Group B^b	60–120^a	Group C^b
Ingredient
Corn	13	22	21	70.65	74.85	73	77.5	75
Fish meal	13	11.2	8.1	13	8.8	6	6.5	3.75
Corn protein	0	0	0	9	9	9	6.5	3.3
Wheat bran	0	0	0	0	5	4.87	7.2	7.8
Whey powder	13.55	18	18	2	0	0	0	0
Milk powder	58	48	47.5	4.4	0	0	0	0
β-conglycinin	0	0	4	0	0	4	0	4
Corn oil	0	0	0	0	1	1	1	1.1
Salt	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2
Premix^c	0.3	0.3	0.3	0.25	0.25	0.25	0.25	0.25
Limestone	0.1	0.3	0.4	0.4	0.7	0.85	0.7	0.85
Calcium phosphate	1.17	0	0	0	0	0.1	0	1.1
L-Lysine	0.68	0	0	0.1	0.2	0.23	0.15	0.15
Cr2O3	0	0	0.5	0	0	0.5	0	0.5
Total	100	100	100	100	100	100	100	100
Nutrient levels
Crude protein	26.37	23.92	23.67	20.82	17.9	17.96	15.73	15.66
DM (MJ/kg)^d	17.23	16.91	16.81	14.38	14.09	14.00	14.01	13.90
Calcium	0.92	0.86	0.78	0.7	0.62	0.59	0.53	0.51
Phosphate	0.75	0.83	0.76	0.66	0.55	0.50	0.50	0.45
Lysine	1.53	2.00	1.94	1.17	0.96	0.95	0.79	0.76
^aThe weight of pigs. ^bGroup A, Group B and Group C mean the experimental periods (pigs were fed diets containing 4% β-conglycinin). ^cThe premix provides following for per kg diet: Fe (as ferrous sulphate) 160 mg; Cu (as copper sulphate) 50 mg; Zn (as zinc sulphate) 160 mg; Mn 40 mg; Se 0.3 mg; I 0.3 mg; VA 45,000 IU; VD3 10,000 IU; VE 60 IU; vitamin K3, 5 mg; vitamin B1, 2 mg; vitamin B2, 15 mg; vitamin B6, 6 mg; vitamin B12, 0.05 mg; folic acid 0.5 mg; biotin 0.1 mg; niacin 40 mg; D-pantothenic acid 25 mg. ^dDE* is calculated value. Other nutrient levels are measured values.

Figure 1.  SDS-PAGE of β-conglycinin. The protein molecular weight marker (low; KDa): phosphorylase b 97.200; bovine serum albumin, 66.400; ovalbumin, 44.300; carbonic anhydrase, 29.000; soybean inhibitor, 201.00; α-lactalbumin, 14.300.

Table 2. Analysis of the immunoreactivity disappearance proportion for β-conglycinin in digesta.

Segments	Physiological stages	Immunoreactivity disappearance proportion
Stomach	A	33.25±0.128^a,1
	B	34.33±0.602^b,1
	C	35.89±0.214^c,1
Upper-jejunum	A	63.41±0.646^a,2
	B	65.49±0.409^b,2
	C	67.02±1.298^c,2
Middle-jejunum	A	83.53±0.589^a,3
	B	85.12±0.286^b,3
	C	86.09±0.401^c,3
Ileum	A	96.73±0.071^a,4
	B	96.85±0.088^a,4
	C	97.07±0.231^a,4
Caecum	A	99.03±0.000^a,5
	B	99.03±0.004^a,5
	C	99.04±0.15^a,5
Effects	Segments	<0.05
	Physiological stages	<0.05
	Segments×physiological stage	<0.05
^a,b,cMean in the same segment followed by different superscripts differs at the p values indicated.
^1,2,3,4,5Mean in the same physiological stage followed by different superscripts differ at the p values indicated.

Figure 2.  SDS-PAGE gels of β-conglycinin in the digesta. Lane 1: the protein molecular weight marker (low; KDa): phosphorylase b 97.200; bovine serum albumin, 66.400; ovalbumin, 44.300; carbonic anhydrase, 29.000; soybean inhibitor, 201.00; α-lactalbumin, 14.300. Lane 2: purified β-conglycinin (added to diets). Lane 3–7: β-conglycinin extracted from the digesta of stomach, upper-jejunum, middle-jejunum, ileum and caecum.