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ARTICLES

Preparation of a bi-hapten antigen and the broad-specific antibody for simultaneous immunoassay of penicillins and tetracyclines in milk

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Pages 273-287 | Received 17 Mar 2011, Accepted 13 Sep 2011, Published online: 26 Oct 2011

Figures & data

Figure 1.  Molecular structures of six penicillins, four tetracyclines and their name abbreviations.

Figure 1.  Molecular structures of six penicillins, four tetracyclines and their name abbreviations.

Figure 2.  Preparation procedure of the bi-hapten conjugates by coupling amoxicillin and chlortetracycline to carrier in turn.

Figure 2.  Preparation procedure of the bi-hapten conjugates by coupling amoxicillin and chlortetracycline to carrier in turn.

Figure 3.  Representative UV scanning diagrams of AOC, CTC, BSA and AOC-BSA-CTC.

Figure 3.  Representative UV scanning diagrams of AOC, CTC, BSA and AOC-BSA-CTC.

Table 1. Specificity and sensitivity of the five antibodies.

Table 2. Results of the optimised ELISA for the PCs and TCs.

Figure 4.  Standard competitive inhibitory curves for four PCs and two TCs by using of coating antigen AOC-OA-CTC and antibody R8 from AOC-BSA-CTC.

Figure 4.  Standard competitive inhibitory curves for four PCs and two TCs by using of coating antigen AOC-OA-CTC and antibody R8 from AOC-BSA-CTC.

Figure 5.  Competitive inhibitory curves for the matrix-matched and the standards of APC, PCV, TC, DC by using of coating antigen AOC-OA-CTC and antibody R8.

Figure 5.  Competitive inhibitory curves for the matrix-matched and the standards of APC, PCV, TC, DC by using of coating antigen AOC-OA-CTC and antibody R8.

Table 3. Recoveries of the 10 PCs and TCs from blank milk (n=6).

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