Changes in quantity of Cry j1, a major cedar pollinosis allergen, in Cryptomeria japonica pollen during spring in Japan

Figures & data

Figure 1. Standard curve of enzyme-linked immunosorbent assay. The horizontal axis indicated concentrations of Cry j1 expressed in ng/ml.

Table 1. Changes in the quantity of Cry j1 in Cryptomeria japonica pollen in Hiroshima Prefecture.

		Quantity of Cry j1 in C. japonica pollen (µg/g)
Tree no.	Parts of a tree	26 February	2 March	3 March	4 March	6 March	7 March	8 March	13 March	14 March
1	Upper part	–	154.0	–	120.5	–	–	64.5	–	–
	Lower part	788.4	140.1		302.0	–	–	120.5	–	–
2	–	–	234.0		222.6	168.3	152.6	213.8	121.3	–
3	–	–	–	–	277.1	132.6	100.1	95.3	–	–
4	–	–	–	242.3	170.1	–	–	146.0	125.8	–
5	–	–	343.8	–	254.2	–	–	196.2	–	51.2

Table 2. Changes in the quantity of Cry j1 in Cryptomeria japonica pollen in Yamanashi Prefecture.

		Quantity of Cry jl in C. japonica pollen (ug/g)
Tree no.	Parts of a tree	25 February	1 March	5 March	10 March	14 March
1	Upper part	591.4	239.6	335.6	261.4	99.2
	Lower part	723.0	367.8	302.0	244.0	105.8
2	Upper part	445.0	283.0	241.0	213.8	83.8
	Lower part	428.2	241.6	268.4	162.6	90.6
3	Upper part	NT	338.8	317.6	240.0	133.8
	Lower part	NT	343.8	254.2	196.2	51.2
Mean±SD	Upper part	–	287.1±49.7	298.1±50.2	238.4±23.8	105.6±25.6
	Lower part	–	317.7±67.0	274.9±24.5	200.9±40.9	82.5±28.2
	Total	546.9±138.4	302.4±55.4*	286.5±37.6*	219.7±36.3*	94.1±27.2*

NT, not tested.

* p<0.05, significance of differences in quantities of Cry j1 in C. japonica at the various sampling dates (February 25 and March 1, 5, 10 and 14) was compared by two-tailed multiple t-test with Bonferroni correction following ANOVA.