Anti-inflammatory effect of new kopetdaghins A, C and E from Dorema kopetdaghense

Figures & data

Figure 1. Chemical structures of kopetdaghin A (1), kopetdaghin C (3) and kopetdaghin E (5) isolated from D. kopetdaghense.

Figure 2. Effect of 10–100 μg/ml of kopetdaghin A (A), kopetdaghin C (B) and kopetdaghin E (C) on the viability of J774A.1 macrophages. Results are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 compared to untreated macrophages.

Figure 3. Evaluation of NO production by J774A.1 macrophages stimulated for 24 h with LPS alone or in combination with 10–100 μg/ml of kopetdaghin A (A), kopetdaghin C (B) and kopetdaghin E (C). Results are shown as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 compared to LPS-stimulated macrophages.

Figure 4. Effect of kopetdaghins A, C and E on LPS-induced iNOS expression. Lysates were prepared from control or LPS (1 μg/ml) stimulated macrophages alone or in combination with kopetdaghins for 24 h. Different lanes were represented as follow: (1) unstimulated macrophages, (2) LPS-stimulated macrophages, (3, 4) stimulated macrophages with 20 and 50 μg/ml of kopetdaghin A, (5, 6) stimulated macrophages with 20 and 50 μg/ml of kopetdaghin C, and (7, 8) stimulated macrophages with 20 and 50 μg/ml of kopetdaghin E.