Advanced search
Publication Cover
Food and Agricultural Immunology Volume 26, 2015 - Issue 4
Submit an article Journal homepage
866
Views
20
CrossRef citations to date
0
Altmetric
Original Articles

Soybean allergen glycinin induced the destruction of the mechanical barrier function in IPEC-J2

Yuan ZhaoKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, P.R. ChinaView further author information
,
Dandan LiuKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, P.R. ChinaView further author information
,
Rui HanKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, P.R. ChinaView further author information
,
Xiaodong ZhangKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun130062, P.R. ChinaView further author information
,
Shiyao ZhangKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, P.R. ChinaView further author information
&
Guixin QinKey Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun130118, P.R. ChinaCorrespondence[email protected]
View further author information
Pages 601-609 | Received 18 Sep 2014, Accepted 26 Nov 2014, Published online: 19 Jan 2015

Figures & data

Figure 1. Metabolic activity of IPEC-J2 cells after 24 h incubation of glycinin measured by MTT assay. Each bar represents 3 independent experiments performed in triplicates ± SD. a, b, c, d each represent two groups for different concentrations which are statistically different from each other.
Figure 1. Metabolic activity of IPEC-J2 cells after 24 h incubation of glycinin measured by MTT assay. Each bar represents 3 independent experiments performed in triplicates ± SD. a, b, c, d each represent two groups for different concentrations which are statistically different from each other.
Figure 2. TEER of IPEC-J2 cells after 0, 6, 12 and 24 h incubation of glycinin. Each bar represents four independent experiments performed in triplicates ± SD.
Figure 2. TEER of IPEC-J2 cells after 0, 6, 12 and 24 h incubation of glycinin. Each bar represents four independent experiments performed in triplicates ± SD.
Figure 3. AP activity of IPEC-J2 cells after 72 h incubation of glycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b each represent two groups for different concentrations which are statistically different from each other.
Figure 3. AP activity of IPEC-J2 cells after 72 h incubation of glycinin. Each bar represents four independent experiments performed in triplicates ± SD. a, b each represent two groups for different concentrations which are statistically different from each other.
Figure 4. TJ proteins immunofluorescence of IPEC-J2 cells after 24 h incubation of glycinin. Representative images of immunofluorescence staining (magnification 200×) are shown.
Figure 4. TJ proteins immunofluorescence of IPEC-J2 cells after 24 h incubation of glycinin. Representative images of immunofluorescence staining (magnification 200×) are shown.
Figure 5. Western blot of TJ proteins of IPEC-J2 cells after 24 h incubation of glycinin. The β-actin is housekeeping protein. Representative Western blots from four independent experiments are shown.
Figure 5. Western blot of TJ proteins of IPEC-J2 cells after 24 h incubation of glycinin. The β-actin is housekeeping protein. Representative Western blots from four independent experiments are shown.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.