Effects of antibacterial peptide on humoral immunity in weaned piglets

Figures & data

Table 1. Composition of experimental diets^a.

Ingredient	(%)
Corn	53.15
Soybean meal (44% crude protein)	25.40
Whey powder	6.00
Fish meal (62.5% crude protein)	4.50
Soybean oil	2.80
Spray-dried porcine plasma (78% crude protein)	3.00
Dicalcium phosphate	2.0
Limestone	1.40
Salt	0.25
L-Lysine HCl	0.15
DL-Methionine	0.35
Premix^b	1.00

^a Calculated nutrient composition (as-fed basis): 3280 kcal of DE/kg, 1.14% lysine, and 0.32% methionine. Analyzed nutrient composition (as-fed basis): 21.1% crude protein, 0.68% Ca, and 0.59% P.

^b Provided the following per kilogram of complete diet: vitamin A, 2250 IU; vitamin D₃, 1050 IU; vitamin E, 16 IU; vitamin K₃, 0.9 mg; vitamin B₁₂, 0.03 mg; riboflavin, 4.5 mg; niacin, 30 mg; pantothenic acid, 25 mg; choline chloride, 400 mg; folic acid, 0.30 mg; thiamin, 2 mg; pyridoxine, 7 mg; biotin, 0.20 mg; Zn, 80 mg; Mn, 22 mg; Fe, 80 mg; Cu, 225 mg; I, 0.50 mg; and Se, 0.25 mg.

Table 2. The effects of ABP on the classical swine fever antibody levels in piglets (optical density ratio) (means ± SD).

	Treatments
Time	Control group	AP group	AMP group I	AMP group II	AMP group III
32 days	0.25 ± 0.02^c	0.28 ± 0.02^b	0.27 ± 0.03^ab	0.31 ± 0.04^ab	0.33 ± 0.04^a
39 days	0.31 ± 0.03^b	0.34 ± 0.02^ab	0.35 ± 0.03^ab	0.36 ± 0.01^a	0.37 ± 0.03^a
46 days	0.34 ± 0.04^b	0.36 ± 0.05^ab	0.38 ± 0.047^ab	0.41 ± 0.05^ab	0.44 ± 0.07^a
53 days	0.30 ± 0.07^c	0.38 ± 0.03^bc	0.39 ± 0.03^abc	0.42 ± 0.04^ab	0.46 ± 0.04^a

Note: Different lowercase letters represent P < 0.05.

Table 3. The effects of ABP on the CH50 levels in piglets (U/mL) (means ± SD).

	Treatments
Time	Control group	AP group	AMP group I	AMP group II	AMP group III
32 days	345.91 ± 10.61^c	435.65 ± 15.86^a	410.34 ± 14.13^b	434.44 ± 14.22^a	445.40 ± 17.48^a
39 days	361.03 ± 22.06^c	459.98 ± 16.60^b	453.43 ± 10.80^b	485.78 ± 17.20^a	503.23 ± 11.32^a
46 days	370.52 ± 14.50^e	487.02 ± 4.38^d	508.70 ± 7.34^c	547.33 ± 11.17^b	571.53 ± 10.16^a
53 days	380.30 ± 20.82^d	503.06 ± 8.55^c	572.47 ± 11.74^b	605.58 ± 21.00^a	612.43 ± 7.48^a

Note: Different lowercase letters represent P < 0.05.

Table 4. The effects of ABP on the immunoglobulin levels in piglets (g/L) (means ± SD).

		Treatments
Items	Time	Control group	AP group	AMP group I	AMP group II	AMP group III
	32 days	3.44 ± 0.14^a	3.49 ± 0.19^a	3.46 ± 0.11^a	3.58 ± 0.54^a	3.66 ± 0.23^a
	39 days	3.63 ± 0.28^b	4.37 ± 0.41^a	3.69 ± 0.14^b	4.50 ± 0.32^a	4.77 ± 0.46^a
IgG
	46 days	3.69 ± 0.33^c	4.90 ± 0.33^a	3.95 ± 0.21^b	4.94 ± 0.42^a	5.20 ± 0.35^a
	53 days	3.47 ± 0.59^b	4.79 ± 0.35^a	3.74 ± 0.53^b	4.89 ± 0.42^a	5.13 ± 0.32^a
	32 days	0.71 ± 0.02^c	0.79 ± 0.02^c	0.78 ± 0.03^c	1.01 ± 0.08^b	1.13 ± 0.09^a
	39 days	0.76 ± 0.04^d	0.93 ± 0.05^c	0.90 ± 0.05^c	1.15 ± 0.08^b	1.27 ± 0.09^a
IgM
	46 days	0.90 ± 0.06^c	1.11 ± 0.05^b	1.00 ± 0.04^c	1.19 ± 0.07^a	1.28 ± 0.010^a
	53 days	0.88 ± 0.04^d	1.10 ± 0.06^b	0.99 ± 0.05^c	1.18 ± 0.06^b	1.25 ± 0.06^a
	32 days	0.52 ± 0.02^a	0.53 ± 0.02^a	0.52 ± 0.01^a	0.53 ± 0.02^a	0.54 ± 0.05^a
	39 days	0.53 ± 0.09^b	0.59 ± 0.08^ab	0.58 ± 0.07^b	0.64 ± 0.04^a	0.68 ± 0.04^a
IgA
	46 days	0.50 ± 0.02^c	0.65 ± 0.04^b	0.64 ± 0.03^b	0.70 ± 0.02^b	0.76 ± 0.05^a
	53 days	0.45 ± 0.04^b	0.67 ± 0.06^a	0.52 ± 0.03^b	0.68 ± 0.04^a	0.73 ± 0.04^a

Note: Different lowercase letters represent P < 0.05.