Preparation of a polyclonal antibody against the cadmium–DTPA complex and its application for determination of cadmium

Figures & data

Figure 1. The dilution of the antiserum.

Table 1. Optimized working concentration of antibody–antigen.

Antiserum	4000	8000	10000	12000	16000	32000
Antigen (μg mL^−1)
1.0	1.550	1.034	0.724	0.868	0.811	0.621
1.3	1.438	1.128	0.910	0.894	0.739	0.575
2.0	1.721	1.124	1.088	0.962	0.773	0.663
2.5	2.313	1.166	1.100	1.168	0.808	0.685
3.0	1.777	1.265	1.183	0.976	0.872	0.654
4.0	1.786	1.171	1.213	1.023	0.834	0.675
5.0	1.818	1.197	1.189	0.997	0.836	0.621
10	1.779	1.233	1.219	0.985	0.814	0.629

Figure 2. Standard inhibition curve for cadmium obtained under the optimized condition of indirect competitive ELISA.

Table 2. CR of other metals in the indirect competitive ELISA for cadmium.

Metal ions	IC50 (μg mL^−1)	CR rate (%)
Cd^2+	1.995	100
Cu^2+	>100	<0.001
Hg^2+	>100	5.8
Pb^2+	>100	<0.001
Zn^2+	>100	<0.001
Mg^2+	>100	<0.001
Ca^2+	>100	<0.001
Fe^2+	>100	<0.001
Ni^2+	>100	<0.001

Table 3. Recovery of cadmium spiked into deionized water.

Cadmium (μg mL^−1)	Added	Detected	Mean recovery % (n = 3)
Sample
Deionized water	0.05	0.51	102
	0.50	0.58	116
	5.00	4.61	92.2