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Food and Agricultural Immunology Volume 27, 2016 - Issue 6
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Articles

In vitro synthesis of a type A trichothecenes complete antigen from T-2 toxin

Pengli LuCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaView further author information
,
Yaling WangCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaCorrespondence[email protected]
View further author information
,
Yapei WangCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaView further author information
,
Chaojin WuCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaView further author information
,
Lijun SunCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaView further author information
,
Defeng XuCollege of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, ChinaView further author information
,
Xiaodong SunCollege of Environment and Resources, Dalian Nationalities University, Dalian116600, ChinaView further author information
,
Jianrong LiFood Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou121013, ChinaView further author information
&
Ravi GooneratneCentre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln7647, Canterbury, New ZealandView further author information
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Pages 870-885 | Received 19 Sep 2015, Accepted 22 May 2016, Published online: 27 Jul 2016

Figures & data

Figure 1. Skeleton structure of type A trichothecenes.

Figure 1. Skeleton structure of type A trichothecenes.

Figure 2. Synthetic pathway towards developing an antigen to type A trichothecenes.

Figure 2. Synthetic pathway towards developing an antigen to type A trichothecenes.

Figure 3. Total ion chromatogram of 3-Ac-T-2 toxin.

Figure 3. Total ion chromatogram of 3-Ac-T-2 toxin.

Figure 4. Mass spectrum of 3-Ac-T-2 toxin.

Figure 4. Mass spectrum of 3-Ac-T-2 toxin.

Figure 5. Total ion chromatogram of 3-Ac-NEOS.

Figure 5. Total ion chromatogram of 3-Ac-NEOS.

Figure 6. Primary mass spectrogram of 3-Ac-NEOS.

Figure 6. Primary mass spectrogram of 3-Ac-NEOS.

Figure 7. Secondary mass spectrogram of 3-Ac-NEOS.

Figure 7. Secondary mass spectrogram of 3-Ac-NEOS.

Figure 8. The 1H-NMR of 3-Ac-NEOS.

Figure 8. The 1H-NMR of 3-Ac-NEOS.

Figure 9. UV spectra of 3-Ac-NEOS (a), 3-Ac-NEOS-HS (b), BSA (c) and 3-Ac-NEOS-HS-BSA (d).

Figure 9. UV spectra of 3-Ac-NEOS (a), 3-Ac-NEOS-HS (b), BSA (c) and 3-Ac-NEOS-HS-BSA (d).

Figure 10. UV Spectra of 3-Ac-NEOS (a), 3-Ac-NEOS-HS (b), OVA (e) and 3-Ac-NEOS-HS-OVA (f).

Figure 10. UV Spectra of 3-Ac-NEOS (a), 3-Ac-NEOS-HS (b), OVA (e) and 3-Ac-NEOS-HS-OVA (f).

Figure 11. Electrophoretic profiles: SDS-PAGE of 3-Ac-NEOS-HS-OVA (lanes 1, 2), OVA (lane 3), BSA (lane 4) and 3-Ac-NEOS-HS-BSA (lanes 5, 6). The molecular marker is in the centre lane (M).

Figure 11. Electrophoretic profiles: SDS-PAGE of 3-Ac-NEOS-HS-OVA (lanes 1, 2), OVA (lane 3), BSA (lane 4) and 3-Ac-NEOS-HS-BSA (lanes 5, 6). The molecular marker is in the centre lane (M).

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