Figures & data
Figure 1. EAY inhibits NF-κB activation induced by LPS. (A) Cell viability assay. (B) NF-κB luciferase reporter assay in RAW264.7 cells. Cells were pretreated with 50 or 100 μg/ml EAY for 1 h and then treated with LPS (10 ng/ml) for an additional 8 h. Cell lysates were prepared and luciferase enzyme activities were determined. Values are expressed as the mean ± SEM (n = 3). *, Significantly different from LPS alone, p < .05 (*). (C) Western blotting for IκBα and β-actin protein. Veh, vehicle; EAY, methylenechloride extracts of Aster yomena.
Figure 2. EAY inhibits COX-2 expression induced by LPS. (A) COX-2 luciferase reporter assay in RAW264.7 cells. Cells were pretreated with 50 or 100 μg/ml EAY for 1 h and then treated with LPS (10 ng/ml) for an additional 8 h. Cell lysates were prepared and luciferase enzyme activities were determined. Values represent the mean ± SEM (n = 3). *, Significantly different from LPS alone, p < 0.05 (*). (B) Western blotting for COX-2 and β-actin protein. Note: Veh, vehicle; EAY, methylenechloride extracts of Aster yomena.
Figure 3. EAY inhibits iNOS expression induced by LPS. (A) iNOS luciferase reporter assay in RAW264.7 cells. Cells were pretreated with 50 or 100 μg/ml EAY for 1 h and then treated with LPS (10 ng/ml) for an additional 8 h. Cell lysates were prepared and luciferase enzyme activities were determined. Values represent the mean ± SEM (n = 3). *, Significantly different from LPS alone, p < .05 (*). (B) Western blotting for iNOS and β-actin protein. (C) NO assay. Values represent the mean ± SEM (n = 3). +, Significantly different from LPS alone, p < .01 (++). Note: Veh, vehicle; EAY, methylenechloride extracts of Aster yomena.
