Figures & data
Table 1. Cross-reaction results of mAb 2G8.
Figure 1. Schematic diagram of the immunochromatographic strip for HDS. The strip is composed of a sample pad, a NC membrane, a PVC backing pad, and an absorption pad.
Figure 2. LC–MS analysis of HDS derivatives.
Figure 3. The UV–Vis spectroscopy of HDS, HDS-HS, protein and conjugates; (a) confirmation of immunogen (HDS-HS–KLH); (b) confirmation of coating antigen (HDS–OVA).
Figure 4. Standard curve of inhibition. Standards 0.02, 0.05, 0.1, 0.2, 0.5, 1, and 2 ng/mL. Each point represents the mean of ±SD of three replicates. IC50 of 2G8 was 0.095 ng/mL, LOD was 0.013 ng/mL, and the linear range of detection was from 0.0258 to 0.356 ng/mL.
Figure 5. Image of detection HDS by immunochromatographic strip in milk. A series of milk samples spiked HDS were subjected to the immunochromatographic strip test. 1 = 0 ng/mL, 2 = 0.2 ng/mL, 3 = 0.5 ng/mL, 4 = 1 ng/mL, and 5 = 2 ng/mL. Cutoff value was 2 ng/mL.
