Figures & data
Figure 1. Chemical structure of DON.
Figure 2. Preparation of 3-HS-DON, (1) 17 mg of l-butaneboronic acid and 5 mg of DON were dissolved in 500 μL of dry pyridine. The mixture was stirred overnight at room temperature. (2) Then, 34 mg of succinic anhydride dissolved in 200 μL of dry pyridine was added to the mixture. After stirring for 3 h at 100°C, the mixture was dried under a nitrogen stream, re-suspended in ethyl acetate and washed three times with water. The organic phase was combined and dried under reduced pressure.
Table 1. Cross-reactivity of DON antibody.
Figure 3. Standard curve of the cd-ELISA for DON: (a) in PBS, (b) in PBS 
, wheat 
, maize ( 
), flour (
), oat 
, rice (I), milk 
and feedstuff 
samples.
Figure 4. Effect of the methanol on the maximum absorbance (▪) and sensitivity of the assay (◆).
Figure 5. Effect of the ionic strength on the maximum absorbance (▪) and sensitivity of the assay (◆).
Table 2. Recovery studies of eight samples at three levels by ELISA (n = 3).
Figure 6. Comparison of ELISA and HPLC for the determination of DON.
