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Articles

Rapid immunological detection of copper ions using fluorescence immunochromatographic assay

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Pages 60-73 | Received 04 Oct 2018, Accepted 23 Oct 2018, Published online: 19 Nov 2018

Figures & data

Figure 1. Chemical structures of p-SCN-Bn-NOTA (a) and Cu-NOTA-OVA (b).

Figure 1. Chemical structures of p-SCN-Bn-NOTA (a) and Cu-NOTA-OVA (b).

Figure 2. The cross-section structure of fluorescence immunochromatographic test strip (a) and the results judgment of immunochromatographic test strips (b).

Figure 2. The cross-section structure of fluorescence immunochromatographic test strip (a) and the results judgment of immunochromatographic test strips (b).

Figure 3. Particle size and appearance of fluorescent microspheres (a) and characteristic absorption peaks of fluorescent microspheres before and after labelling to McAb (b).

Figure 3. Particle size and appearance of fluorescent microspheres (a) and characteristic absorption peaks of fluorescent microspheres before and after labelling to McAb (b).

Figure 4. The T-line and C-line signal of fluorescence immunochromatographic test strips (a) and the standard curve of FICA (b).

Figure 4. The T-line and C-line signal of fluorescence immunochromatographic test strips (a) and the standard curve of FICA (b).

Figure 5. Accuracy and precision test of intra-assay and inter-assay (a), specificity test (b) and stability test (c) of FICA.

Figure 5. Accuracy and precision test of intra-assay and inter-assay (a), specificity test (b) and stability test (c) of FICA.

Table 1. The add recovery rates of copper ions detected by FICA.

Table 2. The digestion recovery rates of the bush branches and soil standard substances.

Figure 6. The copper content detected by FICA and ICP-AES in herbal plants (a) and soil (b) samples. * The copper content of several soil samples beyond the maximum limit standard of 200 mg/kg which regulated in Environmental quality standard for soils (GB15618).

Figure 6. The copper content detected by FICA and ICP-AES in herbal plants (a) and soil (b) samples. * The copper content of several soil samples beyond the maximum limit standard of 200 mg/kg which regulated in Environmental quality standard for soils (GB15618).

Table 3. Comparison of different detection methods for copper ions (Kong et al., Citation2010; Quinn et al., Citation2018; Xing, Feng et al. Citation2013).

Table A1. Detection results of copper content in herbal plants samples.

Table A2. Detection results of copper content in soil samples.