Figures & data
Table 1. Urease activity after anti-urease IgY treatment.
Figure 1. Confirmation of recombinant protein expression (UreA). Notes: Results of SDS-PAGE and western-blot analysis of Urease A. pET32a-UreaseA: 51 kDa. SDS-PAGE, Lane 1,3- Before induction; 2,4- Before induction.
![Figure 1. Confirmation of recombinant protein expression (UreA). Notes: Results of SDS-PAGE and western-blot analysis of Urease A. pET32a-UreaseA: 51 kDa. SDS-PAGE, Lane 1,3- Before induction; 2,4- Before induction.](/cms/asset/72d95024-9560-438c-b886-61a5b5b60453/cfai_a_1617251_f0001_oc.jpg)
Figure 2. Confirmation of recombinant protein expression (UreB). Notes: Results of SDS-PAGE and Western-blot analysis of Urease B protein. pET32a-UreaseB 81 kDa. SDS-PAGE, Lane 1,3- Before IPGT induction; 2,4- After IIPGT induction.
![Figure 2. Confirmation of recombinant protein expression (UreB). Notes: Results of SDS-PAGE and Western-blot analysis of Urease B protein. pET32a-UreaseB 81 kDa. SDS-PAGE, Lane 1,3- Before IPGT induction; 2,4- After IIPGT induction.](/cms/asset/a6e48b74-3b28-4eee-911b-3cf739a326f8/cfai_a_1617251_f0002_oc.jpg)
Figure 3. Detection of egg yolk antibody. Notes: Results of egg yolk antibody detection test by 12% SDS-PAGE.
![Figure 3. Detection of egg yolk antibody. Notes: Results of egg yolk antibody detection test by 12% SDS-PAGE.](/cms/asset/b6a26f56-97f1-4c41-bed3-5f7050f8e549/cfai_a_1617251_f0003_oc.jpg)
Figure 4. Binding identification test for anti-bacteria. Notes: Binding affinity test for egg yolk antibody by 12% SDS-PAGE. M: Protein Marker, 1: H. pylori protein, 2: Anti-H against H. pylori. H. pylori-IgY Binding affinity was confirmed by western-blot.
![Figure 4. Binding identification test for anti-bacteria. Notes: Binding affinity test for egg yolk antibody by 12% SDS-PAGE. M: Protein Marker, 1: H. pylori protein, 2: Anti-H against H. pylori. H. pylori-IgY Binding affinity was confirmed by western-blot.](/cms/asset/c602176b-b7d4-44fe-9922-79666a701925/cfai_a_1617251_f0004_oc.jpg)
Figure 5. Coagulation reaction confirmation test result. Notes: Cohesion test results. IgY sample 40 mg/ml starting, 2-fold dilution, optical microscopy at 200× magnification.
![Figure 5. Coagulation reaction confirmation test result. Notes: Cohesion test results. IgY sample 40 mg/ml starting, 2-fold dilution, optical microscopy at 200× magnification.](/cms/asset/6233da22-246d-4203-9ec6-26979440fa25/cfai_a_1617251_f0005_oc.jpg)
Table 2. Test results for coagulation by IgY concentration.
Figure 6. Urease inhibition result. Notes: Urease activity inhibition confirmation test results. *p < 0.05, **p < 0.01, ***p < 0.001 vs control.
![Figure 6. Urease inhibition result. Notes: Urease activity inhibition confirmation test results. *p < 0.05, **p < 0.01, ***p < 0.001 vs control.](/cms/asset/755605ee-744e-430e-9cae-d78b598b4264/cfai_a_1617251_f0006_ob.jpg)