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Food and Agricultural Immunology Volume 30, 2019 - Issue 1
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Articles

Simultaneous detection of carbofuran and 3-hydroxy-carbofuran in vegetables and fruits by broad-specific monoclonal antibody-based ELISA

Jianqiang Lana State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, People’s Republic of China;b Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education, Hainan University, Haikou, People’s Republic of China;c Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, People’s Republic of ChinaView further author information
,
Mifang Wangc Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, People’s Republic of ChinaView further author information
,
Shang Dingc Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, People’s Republic of ChinaView further author information
,
Yongmei Fanb Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education, Hainan University, Haikou, People’s Republic of China;c Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, People’s Republic of ChinaView further author information
,
Xiaoping Diaoa State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, People’s Republic of ChinaView further author information
,
Qing X. Lid Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, USAView further author information
&
Hongwei Zhaoa State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, People’s Republic of China;b Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education, Hainan University, Haikou, People’s Republic of China;c Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, People’s Republic of ChinaCorrespondence[email protected] [email protected]
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Pages 1085-1096 | Received 31 Jul 2019, Accepted 03 Sep 2019, Published online: 22 Sep 2019

Figures & data

Figure 1. The chemical synthesis procedure of hapten.

Figure 1. The chemical synthesis procedure of hapten.

Figure 2. Standard curve for CBF and 3-OH-CBF by icELISA.

Figure 2. Standard curve for CBF and 3-OH-CBF by icELISA.

Table 1. Hybridoma cell lines that can recognize both carbofuran and 3-OH-carbofuran.

Table 2. Cross reactivities of carbofuran, 3-OH-carbofuran, and related analytes to the mAb2E3 in the icELISA.

Table 3. Average recoveries of carbofuran or 3-OH-carbofuran spiked in long bean, chives, mango and watermelon samples.

Table 4. CBF and 3-OH-CBF in real samples determined by icELISA and UPLC-MS/MS.

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