Identification of immunogenic epitopes of two soybean glycinin proteins in chicken

Figures & data

Table 1. Eggs from seven local sources were used to isolate total IgA, IgM and IgY.

Eggs	Glycinin			Total
	IgM	IgA	IgY	IgA	IgM	IgY
AEW1	0.69	5.22	0.11	11,450	3420	98,330,000
AEW2	0.90	6.33	0.26	6110	760	37,970,000
AEW3	1.19	5.22	0.33	9180	4975	51,690,000
AEW4	0.93	13.63	0.62	35,030	12,430	16,020,000
AEW5	0.90	18.77	1.13	40,695	3070	3,372,000
AEW6	1.30	17.27	1.84	17,330	4325	22,520,000
EBW1	0.32	9.03	0.08	5765	380	130,260,000
EBW2	0.90	8.29	0.26	14,405	4370	112,910,000
EBW4	0.43	2.60	0.38	4135	915	30,580,000
EBW5	0.78	11.23	0.35	9885	855	46,150,000
EBW6	0.79	13.74	0.47	17,680	2680	29,570,000
EBW7	0.62	2.04	0.48	4540	5005	46,100,000
FLW1	0.72	10.10	0.25	9490	2415	108,260,000
FLW2	0.49	10.73	0.28	19,115	1220	42,940,000
FLW3	1.06	11.15	0.63	24,465	3390	32,030,000
FLW4	0.98	7.97	0.21	10,875	1735	60,620,000
FLW5	0.37	12.61	0.40	25,110	2285	26,220,000
FLW6	0.94	20.18	1.44	19,140	1125	2,108,000
HEW1	0.44	3.72	0.19	2575	880	128,930,000
HEW2	1.20	11.93	0.43	7705	1510	40,710,000
HEW3	0.91	4.80	0.26	5185	2130	68,810,000
HEW4	0.49	3.08	0.32	8370	1295	43,440,000
HEW5	0.79	2.80	0.56	1870	1800	78,200,000
HEW6	0.56	5.55	1.63	12,950	4265	1,303,000
NFW1	1.06	14.45	0.24	13,415	1335	123,230,000
NFW2	0.50	13.84	0.19	5425	3605	112,850,000
NFW3	1.22	6.42	0.42	7870	1485	26,590,000
NFW4	1.00	7.03	0.20	8690	2620	32,280,000
NFW5	0.78	6.24	0.35	16,510	3665	30,340,000
NFW6	0.59	3.18	0.16	6655	1645	57,830,000
OVW1	1.56	7.16	0.15	10,875	4695	115,450,000
OVW2	1.14	8.01	0.30	6330	2135	36,870,000
OVW3	0.85	9.31	0.61	20,555	5795	63,640,000
OVW4	0.96	11.63	0.25	20,135	2805	71,900,000
OVW5	1.04	4.08	1.15	13,005	4705	2,400,000
OVW6	0.95	13.70	0.43	46,610	11,645	23,330,000
VFW1	0.71	3.38	0.25	4375	1170	143,050,000
VFW2	0.97	6.30	0.57	2630	1115	100,640,000
VFW3	0.41	2.34	0.49	1220	220	34,770,000
VFW4	0.73	4.58	0.41	2760	615	41,210,000
VFW5	0.47	2.36	0.59	9545	2265	41,360,000
VFW6	0.33	1.72	0.43	2165	835	46,380,000

Notes: The total amounts (ng μl 1) of antibodies are reported. These purified antibodies were used in an ELISA to measure binding of each antibody class to glycinin protein relative to a control serum included in each plate. Igs from eggs in bold were used to identify peptides that bind IgA, IgM and IgY.

Table 2. The correlation between total Ig levels and Ig classes that recognize glycinin is shown above the diagonal.

		Glycinin			Total
		IgM	IgA	IgY	IgA	IgM	IgY
Glycinin	IgM	1.00	0.34	0.18	0.22	0.30	−0.09
	IgA	0.02	1.00	0.36	0.68	0.27	−0.19
	IgY	0.24	0.01	1.00	0.31	0.19	−0.58
Total	IgA	0.14	0.00	0.04	1.00	0.68	−0.41
	IgM	0.04	0.07	0.21	0.00	1.00	−0.23
	IgY	0.55	0.23	0.00	0.00	0.13	1.00

Note: The p value of each correlation is shown in bold below the diagonal.

Figure 1. Alignment of the acidic subunits of two glycinin proteins, A1aBx and A5A4B3. Conserved amino acids are highlighted. Epitopes that bind IgY, IgA and/or IgM in at least three eggs are shown.

Figure 2. Alignment of the basic subunits of two glycinin proteins, A1aBx and A5A4B3. Conserved amino acids are highlighted. Epitopes that bind IgY, IgA and/or IgM in at least three eggs are shown.