2,632
Views
33
CrossRef citations to date
0
Altmetric
Articles

Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay

, , , , , , & show all
Pages 165-175 | Received 15 Oct 2019, Accepted 17 Dec 2019, Published online: 06 Jan 2020

Figures & data

Figure 1. Chemical structures of carbofuran and 3-hydroxy-carbofuran.

Figure 1. Chemical structures of carbofuran and 3-hydroxy-carbofuran.

Figure 2. Schematic illustration of colloidal gold-strip.

Figure 2. Schematic illustration of colloidal gold-strip.

Figure 3. Colloidal gold-strip-based immunoassay of CBF and 3-OH-CBF. Standard solutions of CBF and 3-OH-CBF at 0, 0.25, 0.5, 1, 2.5, 5, 10, and 20 ng/mL were prepared respectively: + means positive and − means negative samples.

Figure 3. Colloidal gold-strip-based immunoassay of CBF and 3-OH-CBF. Standard solutions of CBF and 3-OH-CBF at 0, 0.25, 0.5, 1, 2.5, 5, 10, and 20 ng/mL were prepared respectively: + means positive and − means negative samples.

Figure 4. (A) Colloidal gold-strip-based immunoassay of different source waters with spiked CBF. (B) Influences of tap water, ddH2O, and PBSTG on the colloidal gold-strip based immunoassays for the CBF detection spiked in five different pesticide preparations. N1 and N2 mean pesticide preparations with negative CBF addition, while P1 to P3 are positive samples.

Figure 4. (A) Colloidal gold-strip-based immunoassay of different source waters with spiked CBF. (B) Influences of tap water, ddH2O, and PBSTG on the colloidal gold-strip based immunoassays for the CBF detection spiked in five different pesticide preparations. N1 and N2 mean pesticide preparations with negative CBF addition, while P1 to P3 are positive samples.

Figure 5. Typical colloidal gold-strip based immunoassay of CBF illegal addition in different pesticide preparations: (A) Negative samples (N1 to N9); (B) negative samples with addition of CBF (N1P to N9P); and (C) three positive samples (P1 to P3).

Figure 5. Typical colloidal gold-strip based immunoassay of CBF illegal addition in different pesticide preparations: (A) Negative samples (N1 to N9); (B) negative samples with addition of CBF (N1P to N9P); and (C) three positive samples (P1 to P3).

Table 1. Different pesticide preparations determined by strip test and UPLC-MS/MS.