Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO₂ nanozyme labelling for the detection of sulfadiazine

Figures & data

Scheme 1. Schematic representation of the BELISA procedure.

Figure 1. The FT–IR spectra of the imprinted film after extraction (a), imprinted film before extraction (b), non-imprinted film (c) and sulfadiazine (d).

Figure 2. Effect of different solutions on the BELISA method.

Figure 3. Standard BELISA competition curves for sulfadiazine with concentrations of 0.0512–160 mg/L in methanol.

Figure 4. Cross-reactivity of three kinds of antibiotics.

Table 1. The cross-reactivity (CR) ratio of three kinds of antibiotics.

Antibiotics	Antibiotics structure	IC50 (mg/L)	CR (%)
Sulfadiazine		30	100
Sulfamethazine		152	19.7
Sulfathiazole		98	30.6

Table 2. The recoveries of spiked sulfadiazine in beef samples by determination using the BELISA method.

Sample	Sample content (mg/L)	Spiked level (mg/L)	Analysis result (mg/L, ±SD)	Recovery (%, ±RSD)
Beef	0.13	0.50	0.52 ± 0.01	78.00 ± 2.56
		2.50	2.20 ± 0.40	82.80 ± 1.81
		12.50	11.50 ± 0.50	90.96 ± 4.35