Rapid and ultrasensitive method for determination of aflatoxin M1 in milk

Figures & data

Figure 1. Test strip.

Figure 2. The influence of AFM1-BSA coated amount.

Figure 3. The influence of drying time.

Figure 4. The infuence of room humidity.

Figure 5. The principle of tracer production.

Figure 6. The influence of reaction time with EDC and NHS.

Figure 7. The influence of Goat-Rabbit’s amount.

Figure 8. Application range.

Figure 9. Standard curve.

Table 1. Precision.

Values (μg/kg)	CVs(%)
	Intra-batch	Inter-batch
0.05	4.14	5.75
1	4.25	5.35

Table 2. Placement stability(0.1μg/kg Value).

	Value(origin)	Value(37°C 7D)	Value(4°C 12M)
	0.101	0.082	0.115
	0.107	0.085	0.106
	0.108	0.135	0.098
	0.102	0.157	0.117
	0.107	0.104	0.117
	0.105	0.118	0.098
	0.106	0.126	0.108
	0.113	0.100	0.099
	0.106	0.091	0.108
	0.108	0.122	0.108
Χ	0.106	0.112	0.108
CVs(%)		9.083	5.105

Table 3. Placement stability(1μg/kg).

	Value(origin)	Value(37°C 7D)	Value(4°C 12M)
	0.989	1.001	1.070
	0.883	0.910	1.098
	0.896	0.871	1.282
	0.931	0.870	0.775
	0.872	0.851	0.825
	0.918	0.929	0.819
	0.947	0.804	1.013
	0.984	1.121	0.840
	0.862	0.869	0.984
	1.036	0.869	0.833
χ	0.932	0.910	0.954
CVs(%)		7.924	12.371

Table 4. Limit of quantification.

No.	Value(μg/kg)
1	0.0093
2	0.0108
3	0.0082
4	0.0121
5	0.0067
6	0.0034
7	0.0069
8	0.0058
9	0.0027
10	0.0092

Figure 10. comparison with UHPLC.

Table

Nation	Maximum levels( μg/kg)
China	0.5
Codex Alimentarius Commission(CAC)	0.05
UK	0.05
USA	0.5
Japan	0.5
EU	0.05