Dietary supplementation with fructooligosaccharides ameliorates allergy development following DEHP exposure in mice

Figures & data

Figure 1. Mast cell number in peritoneal lavage fluid. Three groups of mice were intratraperitoneally administered vehicle, ovalbumin (OVA) + di-(2-ethylhexyl) phthalate (DEHP), or OVA + DEHP feeding a diet containing 0 or 2.5% FOS ad libitum from Day 7 to Day 43 (OVA + DEHP + FOS). Peritoneal lavagewas conducted 24 h after the last administration. Differential cell counts were assessed with Diff-Quik staining. Values are the mean ± SE (n = 8). # P < 0.05 vs. OVA + DEHP group.

Table 1. Cellularity in peritoneal lavage fluid and protein levels of interleukin-5, eotaxin, and keratinocyte-derived chemoattractant in peritoneal lavage fluid.

	Neutrophils	Lymphocytes (×10^4/PE)	Eosinophils	IL-5	Eotaxin (pg/PE)	KC
vehicle	2.9 ± 0.2	1.6 ±0.4	0.8 ±0.5	18.7 ±6.1	47.2 ±2.7	18.6 ±6.1
OVA + DEHP	469.0 ±8.6**	6.1 ±2.3 *	71.3 ±10.9 **	798.0 ± 391.1**	530.7 ±96.9**	2482.3 ± 189.9**
OVA + DEHP + FOS	22.1 ±3.0^##	4.1 ±1.0	59.2 ±11.9	240.5 ±91.6^#	140.6 ±31.5^#	37.8 ±14.6^##

Notes: Values are the mean ± SE (n = 8). * P < 0.05 vs. vehicle group, ** P < 0.01 vs. vehicle group, # P < 0.05 vs. ovalbumin (OVA) + di-(2-ethylhexyl) phthalate (DEHP) group, and ## P < 0.01 vs. OVA + DEHP group.