Methods of simultaneous preparation of various active substances from Stichopus chloronotus and functional evaluation of active substances

Figures & data

Table 1. Proximate compositions of Stichopus chloronotus.

Component	Content(oven dry basis)
Protein	79.84
Lipid	4.38
Ash	12.72
Polysaccharides	8.13
Saponins	0.25

Table 2. Sea cucumber polysaccharide, polypeptide, saponin and lipid content and extraction rate.

Item	Content (%)	Extraction rate (%)
Polypeptide	95.64	75.15
Lipid	97.57	86.17
Polysaccharide	65.96	62.32
Saponin	92.13	79.15

Extraction rate = extract mass / effective component mass in sea cucumber body wall

Such as: Sea cucumber polypeptide extraction rate = sea cucumber polypeptide quality / crude protein quality in sea cucumber body wall.

Table 3. Fatty acid analysis of sea cucumber body wall extract.

Fatty acid classification	Fatty acid name	Fatty acid formula	Content(%)
Saturated fatty acid	Myristate	14:0	0.72
	Pentadecylic	15:0	0.65
	Palmitate	16:0	5.06
	Margaric	17:0	0.87
	Stearate	18:0	3.56
	Nonadecanoic	19:0	0.75
	Arachidate	20:0	1.35
	Heneicosanoic	21:0	0.78
	Docosanoate	22:0	0.92
	Lignocerate	24:0	1.16
Monounsaturated fatty acid	Myristoleate	14:1	0.52
	Palmitoleate	16:1	11.82
	Oleate/Elaidate	18:1	10.61
	Eicosenoate	20:1	6.87
	Erucate	22:1	2.01
	Nervonate	24:1	1.27
polyunsaturated fatty acid	Linoleate	18:2	1.26
	Linolenate	18:3	1.31
	Arachidonate	20:4	10.97
	Eicosapentaenoate	20:5	29.65
	Docosahexaenoate	22:6	5.46
SFA	15.82
MUFA	33.10
PUFA	48.65

SFA: saturated fatty acid. MUFA: monounsaturated fatty acid. PUFA: polyunsaturated fatty acid.

Figure 1. Body weight and hepatic index of mice.

Figure 2. Contents of TG, MDA and GSH in serum of mice.

Table 4. Amino acid composition.

Amino acid classification	Amino acid name	Content(%)
EAA	Valine	3.333
	Methionine	1.508
	Leucine	3.843
	Isoleucine	2.677
	Threonine	2.983
	Phenylalanine	2.412
	Lysine	2.098
	Histidine	0.984
NEAA	Arginine	7.282
	Aspartic acid	8.815
	Tyrosine	1.993
	Serine	2.752
	Glutamic	13.631
	Glycine	16.090
	Alanine	7.657
	Cysteine	0.952
	Proline	7.664
Lysine/ Arginine	0.288
EAA	19.802
NEAA	66.835

EAA, essential amino acids; NEAA, nonessential amino acids.