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Research Article

Transcriptomics reveals the potential mechanism of ellagic acid extract from raspberry on wound healing

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Article: 2214709 | Received 21 Feb 2023, Accepted 28 Apr 2023, Published online: 09 Jun 2023

Figures & data

Table 1. The sequences of the primers for real-time PCR.

Figure 1. Electrophoresis of total RNA detection (M, Marker; 1∼3, Control group; 4∼6, EAE group; 7∼9, EAS group; 10∼12, rh-EGF Group).

Figure 1. Electrophoresis of total RNA detection (M, Marker; 1∼3, Control group; 4∼6, EAE group; 7∼9, EAS group; 10∼12, rh-EGF Group).

Table 2. RNA quality assessment.

Table 3. Statistics and quality estimation of RNA-seq reads.

Figure 2. (A) TPM number distribution violin illustration. (B) Different treatment group expression between sample quantity PCA analysis. (C) Venn diagram of differentially expressed genes.

Figure 2. (A) TPM number distribution violin illustration. (B) Different treatment group expression between sample quantity PCA analysis. (C) Venn diagram of differentially expressed genes.

Figure 3. Volcano map of differentially expressed genes between different groups. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 3. Volcano map of differentially expressed genes between different groups. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 4. GO classification statistics. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 4. GO classification statistics. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 5. GO function annotation and function enrichment analysis. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 5. GO function annotation and function enrichment analysis. (A) CTL_VS_EAE, (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 6. KEGG enrichment analysis bubble chart of different treatment groups. (A) CTL_VS_EAE (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 6. KEGG enrichment analysis bubble chart of different treatment groups. (A) CTL_VS_EAE (B) CTL_VS_EAS, (C) CTL_VS_rh-EGF.

Figure 7. Comparison of RNA-Seq and RT-qPCR gene expression levels. (A) SCD-1 (B) FASN (C) SREBP-1c (D) PTTG (E) CDC20 (F) Cyclin A (G) NGFR (H) Fn14 (I) CCL2.

Figure 7. Comparison of RNA-Seq and RT-qPCR gene expression levels. (A) SCD-1 (B) FASN (C) SREBP-1c (D) PTTG (E) CDC20 (F) Cyclin A (G) NGFR (H) Fn14 (I) CCL2.