Topical administration of mangiferin promotes healing of the wound of streptozotocin-nicotinamide-induced type-2 diabetic male rats

Abstract

Purpose

This study identifies the potential use of mangiferin gel to promote wound healing in diabetes mellitus (DM).

Materials and methods

Male rats were rendered diabetes mellitus via intraperitoneal injection of streptozotocin and nicotinamide. Following diabetes development, wound was created at the back of the neck. 1% and 2% mangiferin gel and 1% silver sulphurdiazine (SS) gel (positive control) were applied to the wound for twenty-one (21) days. Fasting blood glucose (FBG) levels were weekly monitored. At the end of the treatment, rats were sacrificed and wound was excised and subjected for histopathological and molecular biological analysis.

Results

No changes to serum FBG levels was noted throughout the period of mangiferin treatment. Albeit, a significant decrease in the size of the wound with increased in the skin thickness of surrounding the wound were observed. Increased expression and distribution of EGF, FGF, TGF-β, VEGF, PI3K, MMP and Nrf2 and decreased expression and distribution of TNFα and NF-κB p65 were observed in diabetic wound treated with topical mangiferin.

Conclusions

Mangiferin has potential to be used as an agent to promote wound healing in diabetic condition.

Keywords:

• Topical
• mangiferin
• wound healing
• diabetes mellitus

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This research is funded by AICTE RPS project grant no. A.V. (3)/AICTE-RPS/2014, India.