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Biocontrol Science and Technology Volume 25, 2015 - Issue 12
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RESEARCH ARTICLE

Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain

Zonglan YuRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
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Liye HeRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
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Li GongRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
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Ganghui HuangRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
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Yao LiuRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
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Ping LiRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. China
&
Aiping ZhengRice Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan611130, P.R. China;Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan625014, P.R. ChinaCorrespondence[email protected]
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Pages 1483-1492 | Received 29 Mar 2015, Accepted 12 Jun 2015, Published online: 29 Sep 2015
 
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Abstract

We characterised a novel holotype cry gene (cry1Hc1) harboured in BN23-5 Bt strain which was isolated from soil samples in Sichuan, China. In this study, the full length of the cry1Hc1 gene was cloned from the strain. The cry1Hc1 gene was inserted into a shuttle vector (pSTK) and expressed in an acrystalliferous mutant Bacillus thuringiensis HD73−. In this transformant, cry1Hc1 was expressed and diamond-shaped parasporal crystals were formed. The resulting insecticidal activity showed that the Cry1Hc1 protein exhibited high larvicidal activity against larvae of Plutella xyllostella and Ostrinia furnacalis with lethal concentration 50 of 55.21 and 118.44 μg/ml, respectively. Sequence analysis of the gene was also performed; the Cry1Hc1 protein retained eight conserved regions commonly found in the existing Cry proteins.

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