Influence of hydraulic retention time on partial nitrification of continuous-flow aerobic granular-sludge reactor

Abstract

This study investigated the effects of hydraulic retention time (HRT) at 12 h, 7.2 h and 2.4 h on partial nitrification efficiency of continuous-flow aerobic granular reactors (CFAGRs) with mature aerobic granules (500±20 mg l⁻¹). At HRT 12 h and 7.2 h, the removal efficiency of both ammonia–nitrogen (NH ₄⁺−N) and nitrite accumulation rate were exceeding 90%. At HRT 2.4 h, NH ₄⁺−N removal efficiency was reduced but most of the conversion efficiency to nitrite was only slightly reduced. At HRT<2.4 h, washout of aerobic granules occurred. In all tests conducted herein, the chemical oxygen demand removal efficiencies exceeded 90%. The clone library results noted the presence of ammonia-oxidizing bacteria belonged to β -Proteobacteria subclass, including 94% of Nitrosomonas europaea and 6% of Nitrosomonas sp. The polymerase chain reaction and denaturing gradient gel electrophoresis results suggested that Alpha proteobacterium, Pseudoxanthomonas mexicana strain, Sphaerotilus natans and Uncultured gamma proteobacterium were responsible for the aerobic granular stability and processing performance. The present CFAGR successfully implemented continuous partial nitrification using aerobic granules at low HRT.

Keywords:

• aerobic granules
• continuous-flow
• clone library
• partial nitrification
• hydraulic retention time

Funding

This work was financially supported by the National Science Foundation of China (No. 51278128).

Supplemental data

of this article can be accessed 10.1080/09593330.2014.881423.