Biohydrogen production from lactose: influence of substrate and nitrogen concentration

Abstract

Hydrogen produced from renewable sources may be considered the energy vector of the future. However, reducing process costs is imperative in order to achieve this goal. In the present research, the effect of nitrogen (N), initial pH and substrate content for starting up the dark fermentative process was studied using the response surface methodology. Anaerobic digested dried sludge (biosolid pellets) was used as the inoculum. Synthetic wastewater was used as the substrate in batch reactors. A decrease in H₂ production was observed with the increase in N and lactose concentrations. This drop was considerably greater when the concentration of lactose was at its lower level. Although the increase in lactose concentration results in a lower H₂ production, the effect of N on the response is attenuated at higher levels of lactose. On the other hand, the effect of initial pH on the fermentation system was not significant. The evaluation on the process under semi-continuous conditions was performed using anaerobic sequencing batch reactors (ASBRs). The process was evaluated at different C/N ratios using synthetic wastewater. Results showed higher hydrogen yields with the gradual decrease in nitrogen content. The addition of cheese whey to the ASBR resulted in a H₂ production rate of 0.18 L H₂ L⁻¹ d⁻¹.

Keywords:

• hydrogen
• dark fermentation
• response surface methodology
• biosolid pellets
• cheese whey

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Junta de Castilla y León, LE182U14, This work was supported by Junta de Castilla y León [grant number LE182U14].