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Articles

Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps

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Pages 154-163 | Received 05 Jul 2018, Accepted 07 Sep 2018, Published online: 19 Sep 2018

Figures & data

Table 1. List and description of collected soil samples.

Figure 1. Photographs of several sites from which soil samples were collected. The samples are described in .

Figure 1. Photographs of several sites from which soil samples were collected. The samples are described in Table 1.

Figure 2. (a) An example of CHROMagar AcinetobacterTM plate (without CR102 supplement for cultivation of carbapenem-resistant bacteria) inoculated with wastewater sample after incubation at 37°C for 48 h. The blue colonies are usually Enterobacteriaceae and red colonies are Stenotrophomonas sp., Acinetobacter sp., Pseudomonas sp., or other Gram-negative bacteria. (b and c) – example of CHROMagar AcinetobacterTM (with CR102 supplement) from here presented study, inoculated with soil sample and incubated at 37°C (b) and 42°C (c).

Figure 2. (a) An example of CHROMagar AcinetobacterTM plate (without CR102 supplement for cultivation of carbapenem-resistant bacteria) inoculated with wastewater sample after incubation at 37°C for 48 h. The blue colonies are usually Enterobacteriaceae and red colonies are Stenotrophomonas sp., Acinetobacter sp., Pseudomonas sp., or other Gram-negative bacteria. (b and c) – example of CHROMagar AcinetobacterTM (with CR102 supplement) from here presented study, inoculated with soil sample and incubated at 37°C (b) and 42°C (c).

Table 2. Numbers of bacteria detected in soil samples (log CFU g−1).

Table 3. The correlations of different groups of bacteria cultivated from soil samples, with each other and with pH and water content (W).

Table 4. List of bacteria with acquired resistance to carbapenems randomly isolated after cultivation at 42°C.