Prediction of size-selective permitted daily exposures for mineral oil mist based on an in vitro study in different scenarios

ABSTRACT

The incidence of DNA damage from exposure to specific types of metalworking fluids has been reported. In this research, size-selective permissible limits to prevent genotoxic damage in A549 cell lines exposed to two types of mineral oil were estimated for the first time using a benchmark dose approach and extrapolated to workers. The comet assay was performed based on Olive and Banath protocol to determine DNA damage. Then, the Benchmark Dose, the 95% lower bound confidence limit BMD, and the 95% upper-bound confidence limit BMD were determined using continuous response data. Finally, the four Benchmark Dose levels reported in the A549 cell line were extrapolated to the human population in occupational settings in two phases. This study showed when determining the permissible limits, the type used or unused, the type of injury, the organ affected in the body and the size of the particles should also be considered.

KEYWORDS:

• Metalworking fluids
• benchmark dose
• permitted daily exposure
• A549 cell line

Acknowledgements

The authors of this article are grateful to the Public Health and Safety School of the Shahid Beheshti University of Medical Sciences and the Department of Biophysics of Tarbiat Modares University for providing laboratory facilities for the present study.

Disclosure statement

No potential conflict of interest was reported by the authors.

Ethics approval

This study approved by the Ethics Committee of Shahid Beheshti University of Medical Sciences with the ethics code IR.SBMU.PHNS.REC.1399.137.

Contributions

Z M: investigation, project administration, writing original draft, and writing – review and editing. R Z: resources, conceptualization, writing the original draft. B H: project administration, writing and editing. P A: Methodology and writing – Original Draft. A K: writing and review. M V: methodology, writing original draft, and writing – review and editing.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/09603123.2023.2196059.

Additional information

Funding

The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by the Public Health and Safety School of the Shahid Beheshti University of Medical Sciences