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International Journal of Food Sciences and Nutrition Volume 68, 2017 - Issue 4
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In Vitro and Animal Studies

Hepatic rhythmicity of endoplasmic reticulum stress is disrupted in perinatal and adult mice models of high-fat diet-induced obesity

Junpei SoedaInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Paul CorderoInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Jiawei LiInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Angelina MouralidaraneInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Esra AsilmazInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Shuvra RayInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Vi NguyenInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Rebeca CarterInstitute for Liver and Digestive Health, University College London, London, UK; View further author information
,
Marco NovelliDepartment of Pathology, University College London, London, UK; View further author information
,
Manlio VinciguerraInstitute for Liver and Digestive Health, University College London, London, UK; ;Fondazione Italiana Fegato, Area Science Park, Basovizza, Trieste, Italy; ;Center for Translational Medicine (CTM), International Clinical Research Center (ICRC), St. Anne’s University Hospital, Brno, Czech Republic; Correspondence[email protected] [email protected]
View further author information
,
Lucilla PostonDivision of Women’s Health, King’s College London, London, UK; View further author information
,
Paul D. TaylorDivision of Women’s Health, King’s College London, London, UK; View further author information
&
Jude A. ObenInstitute for Liver and Digestive Health, University College London, London, UK; ;Department of Gastroenterology and Hepatology, Guy’s and St Thomas’ Hospital, NHS Foundation Trust, London, UKCorrespondence[email protected] [email protected]
View further author information
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Pages 455-466 | Received 11 Jun 2016, Accepted 11 Nov 2016, Published online: 29 Nov 2016

Figures & data

Table 1. Composition of diets.

Table 2. Antibodies for western blotting.

Table 3. Rt-qPCR primers sequences.

Figure 1. Phenotypical, histological and biochemical evidences of NAFLD phenotype in offspring at 6 months: (A) body weight, (B) liver weight, (C) H&E stains of representative liver sections, (D) NAFLD activity score, (E) plasma ALT concentrations and (F) hepatic triglyceride content. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

Figure 1. Phenotypical, histological and biochemical evidences of NAFLD phenotype in offspring at 6 months: (A) body weight, (B) liver weight, (C) H&E stains of representative liver sections, (D) NAFLD activity score, (E) plasma ALT concentrations and (F) hepatic triglyceride content. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

Figure 2. Protein and mRNA concentration of UPR pathways in normal physiological condition (OffCon-SC experimental group; offspring of lean weaned onto standard chow diet) during 24 h. n = 3–4 per experimental time.

Figure 2. Protein and mRNA concentration of UPR pathways in normal physiological condition (OffCon-SC experimental group; offspring of lean weaned onto standard chow diet) during 24 h. n = 3–4 per experimental time.

Figure 3. Protein and mRNA hepatic concentrations of UPR pathways depending on maternal feeding and offspring post-weaning diet during 24 h. n = 3–4 per experimental group/time point; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; *statistical differences (p < .05) between OffCon-SC and OffCon-Ob. #Statistical differences (p < .05) between OffCon-SC and OffOb-Ob.

Figure 3. Protein and mRNA hepatic concentrations of UPR pathways depending on maternal feeding and offspring post-weaning diet during 24 h. n = 3–4 per experimental group/time point; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; *statistical differences (p < .05) between OffCon-SC and OffCon-Ob. #Statistical differences (p < .05) between OffCon-SC and OffOb-Ob.

Figure 4. Inflammation, apoptosis and autophagy assessment by (A) TUNEL staining assay, (B) active caspase-3 staining and (C) phospho-JNK protein concentration measurement. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

Figure 4. Inflammation, apoptosis and autophagy assessment by (A) TUNEL staining assay, (B) active caspase-3 staining and (C) phospho-JNK protein concentration measurement. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

Figure 5. Specific DNA methylation measurement in GRP78 promoter region. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

Figure 5. Specific DNA methylation measurement in GRP78 promoter region. n = 5 per experimental group; values shown as mean ± SEM; OffCon-SC: offspring of lean weaned onto standard chow diet; OffCon-OD: offspring of lean weaned onto obesogenic diet; OffOb-SC: offspring of obese weaned onto standard chow diet; OffOb-OD: offspring of obese weaned onto obesogenic diet; one way ANOVA with Tukey’s post-hoc test for the main comparisons (OffCon-SC versus OffCon-OD; OffCon-SC versus OffOb-OD; OffCon-OD versus OffOb-OD).

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