Transcriptome sequencing and characterization for Kappaphycus alvarezii

Figures & data

Table 1. Summary of transcriptome sequencing and assembly for K. alvarezii unigenes.

Category	Number
Total number of raw reads	77 463 312
Total number of clean reads	61 168 402
G+C content	52.66%
Total number of contigs generated	84 827
Contig size N50 (nt)	723
Average length of contigs (nt)	333
Total number of unigenes generated	28 701
Unigene size N50 (nt)	1697
Average length of unigenes (nt)	901

Table 2. Functional annotation of non-redundant unigenes.

Database	Number of annotated unigenes	Percentage of annotated unigenes (%)
NR	11 456	39.9
Swissprot	8970	31.3
COG	7296	25.4
GO	5394	18.8
KEGG	8975	31.3

Fig. 1. The annotated unigenes (hit) of all unigenes correlates to the length of the unigene. The white bar shows the number of unigenes and the grey bar shows the number of the unigene that can be annotated. By comparing the proportion of the annotated gene within all the unigenes with different length, the result shows that longer unigenes were more likely to have annotation against protein databases.

Fig. 2. Gene Ontology classification of the K. alvarezii transcriptome. A total of 11,456 unigenes with BlastX matches against the Nr database were classified into three main GO categories and 52 sub-categories. The scale on the y-axis indicates the number of unigenes in each category.

Fig. 3. COG functional classification of the K. alvarezii unigenes. A total of 18 616 unigenes were assigned to the 25 COG classifications.