Figures & data
Table I. Plants contain varying numbers of PATs. Data was obtained from TIGR/JCVI (www.tigr.org) and Gene Index (http://compbio.dfci.harvard.edu/tgi/) sequencing projects and represents current numbers based on EST data and hence may not represent the final number for a given species.
Figure 1. ROP10 requires S-acylation for localization to the plasma membrane. Deletion of either or both S-acylated cysteines in the ROP10 C-terminus leads to ROP10 being found largely in the cytoplasm and nucleoplasm indicating that ROP10 requires dual S-acylation for stable membrane association. (A) GFP-ROP10; (B) GFP-ROP10 C199S; (C) GFP-ROP10 C205S; (D) GFP-ROP10 C199,205S. Scale bar represents 10 µm.
Figure 2. CBL1 is N-myristoylated and S-acylated and both modifications are required for membrane association. (A) CBL1-YFP is found at the plasma membrane. (B) CBL1-YFP G2A mutants lacking the N-myristoylated glycine are found in the cytoplasm and nucleoplasm. (C) CBL1-YFP C3S mutants lacking the S-acylated cysteine are found associated with most cellular membranes with a small amount in the cytoplasm and nucleoplasm. This suggests that CBL1 S-acylation requires prior N-myristoylation. Scale bar represents 10 µm.
