Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation

Figures & data

Table

Antibody name	Species origin	Company	No.	Dilution ratio	Antibody recognition size
LC3	Rabbit	CST	#12741	1:1000	14, 16 kDa
GAPDH	Mouse	Santa Cruz	sc-32233	1:2000	36 kDa

Table

Antibody name	company	NO.	Dilution ratio
Rabbit IgG	Santa Cruz	sc-2004	1:5000
Mouse IgG	Santa Cruz	sc-2005	1:5000

Figure 1. A–E respectively on behalf of the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group; 25 µg/mL SN50 group and 50 µg/mL SN50 group. F–J is repeated A–E.

Figure 2. There was no significant difference between group A, B, C and E. However, the expression of LC3 protein was significantly lower in group D, ****p < .05 compared to other cases. The same results appeared in group F–J, the expression of LC3 protein was significantly lower in group I, ****p < .05 compared to other cases.

Figure 3. A–E respectively on behalf of the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group; 25 µg/mL SN50 group and 50 µg/mL SN50 group.

Figure 4. There was no significant difference among group A, B, C and E. However, there were significantly fewer green fluorescent particles in group D, ***p < .05 compared to other cases.