Figures & data
Table S1. Eukaryotic Members of the Bestrophin Family.1
Table S2. Prokaryotic Members of the Bestrophin Family.
Table I. Eukaryotic Members of the Bestrophin Family1.
Figure 1. Average hydropathy (black) and similarity (gray) plots for the eukaryotic bestrophin homologues listed in . The plots are based on the multiple alignment generated with the CLUSTAL X program (see Figure S1 on our website). The AveHAS program (Zhai & Saier [Citation2001a]) was used to derive the plots with a sliding window of 19 residue positions. Peaks 1–4, the strongly conserved hydrophobic peaks, are numbered above the peaks of hydropathy and correspond to putative TMSs 1-4. The two centrally located moderately hydrophobic peaks are labeled M1 and M2. An expanded version of the average hydropathy and average similarity plots for alignment positions 900–1600 can be found on our website (Figure S4).
![Figure 1. Average hydropathy (black) and similarity (gray) plots for the eukaryotic bestrophin homologues listed in Table I. The plots are based on the multiple alignment generated with the CLUSTAL X program (see Figure S1 on our website). The AveHAS program (Zhai & Saier [Citation2001a]) was used to derive the plots with a sliding window of 19 residue positions. Peaks 1–4, the strongly conserved hydrophobic peaks, are numbered above the peaks of hydropathy and correspond to putative TMSs 1-4. The two centrally located moderately hydrophobic peaks are labeled M1 and M2. An expanded version of the average hydropathy and average similarity plots for alignment positions 900–1600 can be found on our website (Figure S4).](/cms/asset/d72603b6-fb61-43f4-b003-3980c7b4f79a/imbc_a_112954_uf0001_b.jpg)
Table II. Prokaryotic Members of the Bestrophin Family.
Figure 2. Average hydropathy (black) and similarity (gray) plots for the prokaryotic bestrophin homologues listed in . The plots are based on the multiple alignment generated with the Clustal X program (see Figure S3 on our website). The AveHAS program (Zhai & Saier [Citation2001a]) was used to derive the plots with a sliding window of 19 residue positions. Peaks 1-4, the strongly conserved hydrophobic peaks, correspond to putative TMSs 1-4. They are numbered above the peaks of hydropathy. The two central moderately hydrophobic peaks are labeled M1 and M2 as indicated in .
![Figure 2. Average hydropathy (black) and similarity (gray) plots for the prokaryotic bestrophin homologues listed in Table II. The plots are based on the multiple alignment generated with the Clustal X program (see Figure S3 on our website). The AveHAS program (Zhai & Saier [Citation2001a]) was used to derive the plots with a sliding window of 19 residue positions. Peaks 1-4, the strongly conserved hydrophobic peaks, correspond to putative TMSs 1-4. They are numbered above the peaks of hydropathy. The two central moderately hydrophobic peaks are labeled M1 and M2 as indicated in Figure 1.](/cms/asset/b34233ec-78f7-4eda-bd9b-8ba37d0433e0/imbc_a_112954_uf0002_b.jpg)
Figure 3. Phylogenetic tree for the eukaryotic bestrophin homologues. The CLUSTAL X program (Jeanmougin et al. [Citation1998]; Thompson et al. [Citation1997]) was used to generate the multiple alignment (Figure S1 on our website) upon which the tree (drawn using the TreeView program; Page, [Citation1996]) was based. Abbreviations of the proteins are as indicated in . Bootstrap values (percentage) from one thousand replications are presented at the nodes. Nodes where no values are indicated have a bootstrap value of 100%.
![Figure 3. Phylogenetic tree for the eukaryotic bestrophin homologues. The CLUSTAL X program (Jeanmougin et al. [Citation1998]; Thompson et al. [Citation1997]) was used to generate the multiple alignment (Figure S1 on our website) upon which the tree (drawn using the TreeView program; Page, [Citation1996]) was based. Abbreviations of the proteins are as indicated in Table I. Bootstrap values (percentage) from one thousand replications are presented at the nodes. Nodes where no values are indicated have a bootstrap value of 100%.](/cms/asset/4999dc32-ba3b-440e-9f18-c206e2b1f680/imbc_a_112954_uf0003_b.jpg)
Figure 4. Phylogenetic tree for the prokaryotic bestrophin homologues. The CLUSTAL X program (Jeanmougin et al. [Citation1998]; Thompson et al. [Citation1997]) was used to generate the multiple alignment (Figure S3 on our website) upon which the tree (drawn using the TreeView program; Page, [Citation1996]) was based. Abbreviations of the proteins are as indicated in . Bootstrap values (percentage) from one thousand replications are presented at the nodes.
![Figure 4. Phylogenetic tree for the prokaryotic bestrophin homologues. The CLUSTAL X program (Jeanmougin et al. [Citation1998]; Thompson et al. [Citation1997]) was used to generate the multiple alignment (Figure S3 on our website) upon which the tree (drawn using the TreeView program; Page, [Citation1996]) was based. Abbreviations of the proteins are as indicated in Table II. Bootstrap values (percentage) from one thousand replications are presented at the nodes.](/cms/asset/eff3d8e8-a75f-40f8-a607-64dd293b94d1/imbc_a_112954_uf0004_b.jpg)