Figures & data
Figure 1. Schematic representation of the bisulphite PCR technique. Cytosines (C) but not methylated cytosines (Cm) are deaminated to U by bisulphite, under appropriate conditions (Hayatsu Citation2008). When clones derived from PCR-amplified bisulphite-treated DNA were sequenced, the only cytosines present in the sequence represent those that were methylated in the original sample: all other cytosines were converted to thymine.
Figure 2. The results of searching for CpG Islands in exon-1 of the HOXC-8 gene. The CpG Island is located at 48–373 bp. Five pairs of primers for bisulphite sequencing PCR are also shown.
Figure 3. The schematic diagram of HOXC-8 and the region amplified by Bisulphite-PCR. (A) The schematic diagram of the HOXC-8 gene of Mongolian sheep. (B) A 293 bp region in exon-1 of the HOXC-8 gene amplified by Bisulphite-PCRs. The CpG sites in exon-1 of the HOXC-8 gene of Mongolian sheep (GenBank Accession No. EU817489) are marked in italics and numbered. The primer is underlined.
Figure 4. Methylation profiles of CpG dinucleotides of exon-1 of the HOXC-8 gene in normal Mongolian sheep (N) and multi-vertebrae Mongolian sheep (T). Open and closed circles indicate non-methylated and methylated CpG sites, respectively.
Figure 5. The average percentages of methylated DNA in HOXC-8 exon-1 in normal and multi-vertebrae Mongolian sheep.
