An outbreak of acute Trypanosoma evansi infection in crossbred cattle in Punjab, India

Figures & data

Figure 1.  Blood film showing actively multiplying stages and micrometric measurements of Trypanosma evansi (Giemsa×100X).

Table 1. Sero-biochemical and haematological findings of acute clinical cases T. evansi.

Parameters	Case no. 27	Case no. 49	Case no. 50	Mean±SE	Ref. range
Total Proteins g/dl	8.1	8.9	9.2	8.73±0.27	6.7–7.4
Albumin g/dl	4.5	3.1	2.8	3.46±0.43	3.0–3.5
BUN mg/dl	7.0	12	14	11.0±1.70	7.8–25
Total Bilirubin mg/dl	1.2	1.6	2.9	1.9*±0.42	0–0.8*
Creatinine mg/dl	0.9	0.8	1.6	1.1±0.21	0.6–1.8
Alkaline Phosphotase unit/L	23.0	28	20	23.6±1.91	0–38
Cholesterol mg/dl	238	206	245	229.6*±9.80	62–193*
Hb (g/dl)	9.5	8.8	9.0	9.1±0.17
TLC per µl	6900	6400	8800	7366.6±596
Neutrophil% (absolute)	4* (276)	60* (3840)	24 (2112)	29.3±13.37 (2076±840)
Lymphocyte (absolute)	92 (6348)	36 (2304)	73 (6424)	67.0±13.42 (5025±1111)
Monocytes% (absolute)	4 (276)	4 (256)	3 (264)	3.67±0.27 (265±5)
Note: *p≤0.05.

Figure 2.  PCR amplified product 227 bp specific to T. evansi.M, Marker ladder of 100 bp; Lane 1, 2 and 3, Positive sample; Lane 4, Negative sample; Lane 5, Negative control.