Tissue specific expression of Pax3/7 and MyoD in adult duck tissues

Figures & data

Table 1. Primers for real time PCR.

Primers	Genebank number	Sequences	Product length (bp)	Annealing temperature (°C)
Pax3	JQ070187	CTATTGCCTACCCAGCACCAG	133	60
		GGTCAGAAGTCCCATTACCTG
Pax7	JQ070188	GAGTTCAGGTGTGGTTCAGCA	169	60
		GAAATGGTGGTGGTTGGGTAG
MyoD	FJ374143	GCAACGCCATCCGCTACAT	85	65
		GCAATCAAGGCTGGAAACAACA
GADPH	AY436595	AAGGCTGAGAATGGGAAAC	254	60
		TTCAGGGACTTGTCATACTTC
β-actin	EF667345	GCTATGTCGCCCTGGATTTC	168	60
		CACAGGACTCCATACCCAAGAA

Figure 1.  The relative mRNA expression level of MyoD in duck different organs and tissues.

Note: Blue bar, expression level in male duck; red bar, expression level in female duck; green bar, the means of male and female. The different letter on each bar means significant different (p<0.05).

Figure 2.  The relative mRNA expression level of Pax3 in duck different organs and tissues.

Note: Blue bar, expression level in male duck; red bar, expression level in female duck; green bar, the means of male and female. The different letter on each bar means significant different (p<0.05).

Figure 3.  The relative mRNA expression level of Pax7 in duck different organs and tissues.

Note: Blue bar, expression level in male duck; red bar, expression level in female duck; green bar, the means of male and female. The different letter on each bar means significant different (p<0.05).

Table 2. Correlation analysis of Pax3/7 and MyoD in 13 tissues.

Tissue		MyoD	Pax3	Pax7	Tissue		MyoD	Pax3	Pax7
Leg muscle	MyoD	1	0.499	.987**	Small intestine	MyoD	1	0.318	0.142
	Pax3		1	0.561		Pax3		1	.887**
	Pax7			1		Pax7			1
Breast muscle	MyoD	1	.931**	.981**	Stomach	MyoD	1	0.21	.890**
	Pax3		1	.983**		Pax3		1	0.105
	Pax7			1		Pax7			1
Heart	MyoD	1	.957**	.931**	Skin fat	MyoD	1	0.051	.989**
	Pax3		1	.990**		Pax3		1	0.022
	Pax7			1		Pax7			1
Liver	MyoD	1	0.511	.819**	Abdomen fat	MyoD	1	−0.253	−0.086
	Pax3		1	0.539		Pax3		1	.623*
	Pax7			1		Pax7			1
Spleen	MyoD	1	.992**	.994**	Brain	MyoD	1	.973**	.943**
	Pax3		1	.999**		Pax3		1	.987**
	Pax7			1		Pax7			1
Lung	MyoD	1	0.168	.814**	Pancreas	MyoD	1	0.438	0.595
	Pax3		1	−0.137		Pax3		1	.696*
	Pax7			1		Pax7			1
Kidney	MyoD	1	.647*	0.052
	Pax3		1	.620*
	Pax7			1
Note: **, Correlation is significant at the 0.01 level. *, Correlation is significant at the 0.05. Pax, Paired box.