Calpastatin polymorphism in Afshari sheep and its possible correlation with growth and carcass traits

Figures & data

Table 1. Alleles and genotype frequency of calpastatin gene in Afshari sheep papulation.

		Genotype frequency (%)			Alleles frequenty (%)
Gene	Samples number	MM	MN	NN	M	N
Calpastatin	51	57	33	10	74	26
Note: M and N are alleles and MM, MN and NN are genotype.

Figure 1.  L: one kb marker (SMO311), -ve: negative control, 1 to 5: PCR amplification of a partial calpastatin gene in Afshari sheep.

Figure 2.  Restriction digestion pattern for calpastatin gene (Cast1) with MspI. The 622 bp fragment was termed the M allele and the 336 and 286 bp fragments were termed the N allele. Assigned genotypes label each lane. L is 100 bp marker.

Table 2. Phenotypic records for carcass and growth traits with corresponding means and SD for each genotype.

Traits	Genotype
	MM µ±SD	MN µ±SD	NN µ±SD
Birth weight kg	5.23±0.55	4.99±0.7	5.51±0.75
Weaning weight kg	33.41±8.74	32.4±6.29	38.05±5.64
6 month weight kg	40.67±6.34	39.25±7.19	44.2±4.49
9 month weight kg	44.1±6.69	43.95±8.05	47.7±5.37
Pre finishing weight kg	44.75±6	41.86±5.26	44.15±4.23
Post finishing weight kg	65.14±6.41	63.44±5.2	63±5.2
Birth-weaning weight gain kg	28.9±5.57	27.41±5.98	32.54±5.14
Weaning-6 weight gain kg	6.53±2.5	6.85±2.13	6.15±2.89
6–9 weight gain kg	3.53±2.4	3.95±3.14	3.5±2.22
Pre-post finishing weight gain kg	20.39±2.62	20.71±2.8	19.82±1.76
Backfat thickness pre finishing, mm	1.58±0.39	1.39±0.22	1.66±0.33
LM thickness pre finishing, mm	17.13±3.17	16.37±4.86	17.33±4.12
LM area, mm^2	998.89±108.11	993.14±131.53	1018±125.47
Backfat thickness post finishing, mm	2.08±0.37	2.14±0.32	1.78±0.44
LM thickness post finishing, mm	20.48±2.36	20.17±1.88	20.78±1.46
Pre-post finishing backfat thickness gain, mm	0.64±0.31	0.75±0.44	0.64±0.17
Pre-post finishing LM gain, mm	3.76±2.96	3.8±1.97	4.21±0.7