Characterisation of 57 novel microsatellite markers from the goose (Anser cygnoides) genome

Figures & data

Table 1. Characterisation of 57 polymorphic microsatellites loci isolated from Anser cygnoides.

Locus/accession no.	Repeat motif	Primer sequence (5′–3′)	ASR (bp)	T a(°C)	N a	H o	H E	P-values
ZAAS001/GU130819	(AC)11	F: TCTTCATGGTCTTTGGCAGA; R: TTTTCGCAGATTGTGTGGAG	184–205	52.7	6	0.65	0.73	0.164
ZAAS004/GU130822	(AC)23	F: TCCCAGGTTCAGTACACACG; R: CGACGATCTGCTCATTTTGA	124–138	52.7	4	0.39	0.62	0.001
ZAAS006/GU130824	(AC)10AT(AC)13	F: GACACACTGCTGCCACTTTC; R: TCCAGTGCGTAAGTGCTGTT	198–212	52.7	3	0.55	0.60	0.020
ZAAS013/GU130830	(AC)23 … (CA)8	F: GTCGACGATCCTACTGTTAAGG; R: CAAATCGTGTAATGGGCAAT	152–172	52.7	3	0.43	0.54	0.202
ZAAS015/GU130832	(GT)13GA(GT)10	F: CATGTTTCAGCTCCAGCAAA; R: GCAAATTAAGCGAGATGCTG	221–245	54.6	3	0.16	0.32	0.003
ZAAS018/GU130835	(GT)21 … (TG)5 … (TG)5 … (GT)10	F: GGGTGGGAGGGTAGGATAAG; R: CACACACACCCACACACACA	378–416	60.3	6	0.49	0.49	0.915
ZAAS020/GU130837	(TG)11	F: CTACCCCACCCATGAGGATT; R: AGAGAGGTCAGCGTGCTTGT	246–264	56.5	10	0.50	0.54	0.516
ZAAS023/GU130840	(AC)8	F: GAGATAAATGCACGGGCTTC; R: ACCAGGGAGGAGGACAAGAT	148–163	54.6	4	0.50	0.73	0.008
ZAAS025/GU130842	(CA)6	F: AAATGCATGGGCACTTCTG; R: AGGGGGCTGTAAATGGAAAT	172–184	54.6	3	0.26	0.47	0.050
ZAAS030/GU130847	(CA)6 … (CA)9	F: TTAACCCCTCCTCTCCCTTC; R: TCTCCGTGAAAAGACCATCC	248–268	54.6	5	0.28	0.56	0.015
ZAAS033/GU130850	(CA)18	F: GTTTGTGCCCTGCATTTGTA; R: GGTGGGAGGTTTTGGAGAGT	385–421	54.6	4	0.32	0.59	0.009
ZAAS035/GU130852	(CA)20	F: TGGCTTATTGCACACATTCC; R: GCTGCTGATCGCTACAGATG	358–378	54.6	6	0.72	0.64	0.504
ZAAS036/GU130853	(TG)17	F: TCCCAGCTTCACTCCTTTTC; R: GTGGTGTTCTTGCGGTGTAG	195–206	54.6	3	0.39	0.64	0.009
ZAAS038/GU130855	(GT)8	F: GGCAAGCTGTATGGTGGTTT; R: GTGTGGTCAGCAAACCAATG	172–182	60.3	2	0.06	0.05	0.072
ZAAS039/GU130856	(GT)9	F: GGACATGGGGAGTGAAACAT; R: CAGTCTAGCTCGTCCCTGCT	350–386	56.5	4	0.82	0.66	0.336
ZAAS040/GU130857	(CA)5GA(CA)5	F: CTTGGGCACGTGGACTCT; R: GAGCTGTGCATGGGGACA	230–248	51.0	4	0.65	0.54	0.017
ZAAS041/GU130858	(GT)8CTC(TG)6C(TG)6	F: GATGCACGGATACATGTTGG; R: CCCAGGAGGGAGAAACTACC	385–406	60.3	5	0.33	0.58	0.017
ZAAS050/GU130867	(GT)11	F: CTGGGGAAAAGGGAGAAGGT; R: ATAGCATCCCTCAGCCACAT	250–270	56.5	6	0.66	0.74	0.306
ZAAS052/GU130869	(TG)10C(GT)6	F: TAGCCTGGAGCTAGGACTGC; R: AAAGGTGCTTGGGATACGTC	192–208	60.3	3	0.38	0.59	0.223
ZAAS053/GU130870	(GC)6(AC)6GTGTG(CA)11	F: ATTCTTGGGCTGTGTGAAGC; R: GCTCCCCAAAAAGCACAC	386–416	52.7	3	0.50	0.62	0.032
ZAAS054/GU130871	(AC)5AA(AC)24	F: CCCCACACCCCCAAAATA; R: AAGGCTAGTTTGCCACAGGA	245–268	60.3	6	0.82	0.78	0.768
ZAAS055/GU130872	(AC)12	F: TGCAATCCATTGCTGGTG; R: GATGTGCAAGTGTGTGGTCA	204–224	56.4	5	0.72	0.75	0.086
ZAAS060/GU130877	(GT)10 … (GT)10	F: GAATACAGCCCTGCATTGAAA; R: TCTCCCACCAGTCCTCTGTT	374–402	52.7	4	0.04	0.52	0.001
ZAAS061/GU130878	(GT)7 … (GT)14	F: CTCCACAGCAGCAAGGTAGC; R: CGTTAGCATCTCCCACATTG	372–395	52.7	5	0.42	0.60	0.006
ZAAS064/GU130881	(AC)7	F: TAGCAGAAGGATTCCCCTCA; R: ATGCTGCTTTGGTTTGGATT	166–182	58.4	3	0.22	0.43	0.024
ZAAS071/GU130887	(TG)18	F: TGTAACTGCCATCCCAAACA; R: AGGGAGTGACAGTGTAGGTGGT	225–258	56.5	7	0.62	0.68	0.464
ZAAS074/GU130890	(TG)10	F: GGCTTCACGTGAGCAGACTT; R: CAAAAGCTGATGGAGGGAAC	185–195	52.7	6	0.27	0.62	0.001
ZAAS075/GU130891	(TG)8	F: ACCATGGCCCATTATTGAAG; R: GTGCACTTTGCAGGCTTATG	178–203	58.4	3	0.32	0.62	0.008
ZAAS079/GU130895	(TG)10	F: CTTTGGGGGAAGTGGTCTTT; R: TGTCCTGTGTAGTGCCCTGT	158–172	58.4	3	0.17	0.50	0.002
ZAAS093/GU130909	(GT)7 … (GT)5	F: CCAGGGTTTCGGTGTCTTG; R: ACACGTGCCAATGCAGAG	380–420	64.0	3	0.33	0.28	0.315
ZAAS096/GU130912	(AC)18	F: GACCCCGTCTACATGGCTTA; R: CTTCCTTGCTTCGTCCTTGT	412–438	56.5	2	0.20	0.48	0.013
ZAAS113/GU130929	(CA)9	F: CATGCACCCAACCCTTTAAT; R: CTGCCTCTTGGAGTTTCCTG	252–260	64.0	3	0.32	0.64	0.034
ZAAS121/GU130937	(CA)6 … (CA)16	F: GTTCCACCCCACTCCTCTCT; R: CCACCCAGGTTAAAATGCTG	200–218	64.0	5	0.89	0.74	0.143
ZAAS128/GU130944	(GT)8	F: TGCAGAAGAGGGCAAGTTTT; R: CACTGTTGCTGGAGTTCTGC	382–390	60.3	3	0.12	0.38	0.001
ZAAS134/GU130950	(GT)7	F: GAATGCAACACAAACGGTGA; R: CCAGAAGCAACAGAAGAGCA	148–180	56.5	5	0.43	0.57	0.205
ZAAS137/GU130953	(GT)8	F: TGGCATTGATTGACTGGAAC; R: AGATCACGTAAACCCCTCCA	264–280	64.0	5	0.16	0.41	0.012
ZAAS139/GU130955	(AC)7	F: ATGAGTGGCATTTTGCTGTG; R: TCGACGATCTTATTTCTTAGTCTCA	308–324	54.6	3	0.17	0.30	0.051
ZAAS141/GU130957	(GT)5	F: TGCAGCTGGGATTTATGACA; R: TACTCCCATGGTGCAAGGTT	250–270	58.4	5	0.22	0.64	0.001
ZAAS142/GU130958	(TG)5	F: CATAGGGGCCAATGAACTTG; R: GCCAACCTGCAAACTCTTGT	240–260	58.4	3	0.67	0.72	0.012
ZAAS144/GU130960	(AC)17	F: GGCCACATTCACTCGTCTTT; R: TAGCACCTCGGAGGGTCA	290–318	56.5	5	0.42	0.57	0.146
ZAAS146/GU130962	(TG)9	F: TTCGCAGCACGAAGATAGTG; R: TGTCAGCAGGACATCTGGAG	195–210	64.0	2	0.12	0.45	0.001
ZAAS149/GU130965	(GT)8	F: TCCCTTGTGCTCTACTCCTCA; R: CTGTGCCATGCCACTACAG	178–202	56.5	7	0.67	0.73	0.154
ZAAS150/GU130966	(GT)7	F: ATCTGTCACGGGGAGCAA; R: ACACCCACCTGAGCACCTAC	196–203	60.3	6	0.65	0.78	0.348
ZAAS151/GU130967	(AC)6	F: CGATCCTTCTCCCTCTCTCC; R: CGCTTGCTATCTAACTGCTTGG	170–184	54.6	2	0.33	0.48	0.165
ZAAS152/GU130968	(GT)9	F: ACTGTAGCGCTCGGTTGTGT; R: ACCTGATGCCTTTCTGAGGA	178–204	58.4	5	0.76	0.73	0.565
ZAAS153/GU130969	(CA)8	F: GAAGGGCTCATCACCAGAAC; R: CAGGGTTCGTAGCCTCATCT	204–230	56.5	3	0.33	0.62	0.007
ZAAS154/GU130970	(TG)7	F: CGTGTCATGCTCAAAAGGAG; R: TGCTTCTCATGGAAACAACG	198–218	56.5	3	0.12	0.11	0.807
ZAAS158/GU130974	(CA)7	F: TCACTGCCAAACATCCTGAA; R: GTCTCGTGGTCAGTCCAGGT	169–186	52.7	3	0.21	0.48	0.001
ZAAS168/GU130984	(TG)16(GA)20	F: GCCCTTTGCATCGTATCTTC; R: GTGCCCATACACCCTCGTTA	202–220	58.4	3	0.56	0.60	0.260
ZAAS169/GU130985	(AC)8	F: AGTTTGAAAGGCAGCACTGG; R: TTTGACCAGAATTTTGACACTGA	216–230	60.3	3	0.38	0.56	0.001
ZAAS172/GU130988	(AC)8 … (CA)17	F: TGTCCCTGTCCCTCACAGAT; R: ATCCCCACATCTCTGACACC	192–210	52.7	4	0.60	0.70	0.780
ZAAS175/GU130991	(TG)11 … (TG)9	F: TGAGTAGTGGGGTCCCTGAA; R: CCCATCTGCTAGTCCAGCTT	168–180	58.4	3	0.12	0.53	0.001
ZAAS177/GU130993	(AC)13	F: GAAAGCTCCCTGATGCTGTT; R: CCTCCATGGGAATGTTTTCT	182–200	56.5	2	0.21	0.34	0.019
ZAAS178/GU130994	(CA)11CG(CA)11	F: GTCATATGGCCTGCACTGTC; R: TGGTCACTGTGCTTGACTTTG	204–218	56.5	6	0.72	0.68	0.275
ZAAS181/GU130997	(GT)11	F: CAGAACAGGGAAGGGATGTG; R: TTCACATCTGCAGGTTCAGG	200–220	58.4	4	0.50	0.54	0.724
ZAAS182/GU130998	(TG)8	F: TTCTTTTCCCCTGTAAGGTTCA; R: CAAGGGGAAACACTTCTTGC	180–204	51.0	3	0.10	0.12	0.806
ZAAS197/GU131013	(CA)5 … (CA)5	F: GTCAACACCGTTCGTTCATC; R: TTGCTTCATGGTTTGCAGAG	196–216	51.0	3	0.20	0.47	0.001
ASR, detected range of allele sizes; Ta, annealing temperature; Na, number of alleles; HO, observed heterozygosity; HE, expected heterozygosity; P-value, probability of being Hardy–Weinberg equilibrium.