mRNA expression, polymorphism of prolactin receptor gene and their association with prolificacy in Lezhi black goats

Figures & data

Table 1. Primers of goat PRLR gene designed for PCR-SSCP analysis.

Primers	GenBank acc. nos.	Primer sequences (5′–3′)	Annealing (°C)	Product size (bp)
P1	AF091870	F: TGTCTGAAAAGTGTGATGAA	52.0	233
		R: AGCAATGTTGTGGTAAGAATA
P2	AF041257	F: CTTACCACAACATTGCTGACG	53.6	231
		R: GTTTAGCAGAGAACAAGGGGG

Figure 1. PRLR mRNA expression in ovary, liver, pituitary, and endometrium of the Tibetan goat and Lezhi black goat (n = 6), as determined by real-time PCR. Values are normalized with the values for β-actin and are expressed as mean ± SEM (standard error of the mean). Different letters in the same tissue indicate the difference between breeds (P < 0.05).

Figure 2. Polymorphism of the exon 10 of PRLR gene in Lezhi black goats identified by using polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis using primer 2. 1, 2: AB genotype; 3, 8: AA genotype; 4: CD genotype; 5–7: AD genotype.

Table 2. Genotype and allele frequencies based on PCR-SSCP analysis of the exon 10 of PRLR gene at the P2 locus and litter size of different genotypes in Lezhi black goats.

Genotype	Number	Genotype frequency	Allele	Allele frequency	Litter size (mean ± SE)
AA	21	0.136	A	0.487	2.17 ± 0.12
AB	17	0.110	B	0.055	1.91^a ± 0.20
CD	25	0.162	C	0.081	2.13 ± 0.15
AD	92	0.594	D	0.377	2.26^b ± 0.06

Note: Values with different superscripts at the same locus differ significantly at P < 0.05.