Polymorphisms of osteopontin gene and their association with placental efficiency and prolificacy in goats

Figures & data

Table 1. The litter size distribution of three goat breeds.

	Litter size
Breed	Single	Twin	Triplet	Quadruplet	Quintuplet	Total litters
Dazu Black	31	46	15	1	1	94
Lezhi Black	15	7	3	2	/	27
Hexi Cashmere	48	2	/	/	/	50

Note: /, no such sample was collected or recorded.

Table 2. Primer sequence, product size, amplified region and annealing temperature used in analyses of the goat OPN gene.

Name of primers	Primer sequence (5′–3′)	Product size/bp	Product position	Annealing temperature
P1	F: AAAGGTAGATGAAGAGGTT	253	Promoter region	49°C
	R: CTGAAGGACTGGCTTAGAT
P2	F: GAGTGCTTCCCTTCCTA	446	Exon 7	53°C
	R: TCTCCCACCCTGCTTTA

Table 3. The comparison of kid, litter and placenta weights, and placental components among three goat breeds.

	Dazu Black	Lezhi Black	Hexi Cashmere
Number of litters	94	27	50
Mean litter size	2.21^A	2.05^A	1.08^B
Litter weight (g)	4139 ± 1647^A	3850 ± 2260^A	2368 ± 428^B
Placenta weight (g)	449.45 ± 174.51^A	395.81 ± 201.01^A	271.88 ± 67.49^B
Placental efficiency	9.75 ± 3.59	10.21 ± 3.70	9.20 ± 2.67
Kid weight(g)	2186.1 ± 608.47^a	2384.6 ± 483.21^b	2276.6 ± 439.9^ab
No. of cotyledons per placenta	60.42 ± 31.44^A	59.69 ± 32.48^A	119.73 ± 30.4^B
No. of small cotyledons	5.56 ± 7.66^A	9.47 ± 9.93^A	53.36 ± 34.77^B
No. of medium cotyledons	48.03 ± 29.25^a	43.22 ± 27.57^a	66.09 ± 17.87^b
No. of large cotyledons	7.15 ± 6.55^A	7.00 ± 5.42^A	0.27 ± 0.9^B
Cotyledon density	0.29 ± 0.15^A	0.30 ± 0.14^A	0.63 ± 0.27^B
Cotyledon efficiency	40.52 ± 23.71^a	42.16 ± 27.40^a	23.71 ± 11.10^b

Note: Means with different superscripts of capital letters in the same trait are most significantly different (P < 0.01); different superscripts of small letters mean significant difference (P < 0.05).

Table 4. The comparison of kid, litter and placenta weights, and placental components among three goat breeds with the litter size is single.

	Dazu Black	Lezhi Black	Hexi Cashmere
Number of litters	31	15	48
Litter weight (g)	2575 ± 657^a	2506 ± 501^ab	2318 ± 390^b
Placenta weight (g)	314.13 ± 88.01^a	289.33 ± 96.19^ab	267.47 ± 66.11^b
Placental efficiency	8.43 ± 1.20	9.30 ± 2.79	9.19 ± 2.72
No. of cotyledons	111.68 ± 31.9	94.44 ± 27.27	121.90 ± 31.13
No. of small cotyledons	12.04 ± 11.77^A	15.56 ± 11.29^A	57.80 ± 33.21^B
No. of medium cotyledons	95.00 ± 30.31^A	76.11 ± 18.71^A	63.80 ± 17.05^B
No. of large cotyledons	4.64 ± 7.32	2.78 ± 3.07	0.30 ± 0.95
Cotyledon density	0.40 ± 0.18^A	0.33 ± 0.10^A	0.65 ± 0.27^B
Cotyledon efficiency	25.17 ± 12.99	27.69 ± 13.02	22.92 ± 11.37

Note: Means with different superscripts of capital letters in the same trait are most significantly different (P < 0.01); different superscripts of small letters mean significant difference (P < 0.05).

Figure 1. PCR-SSCP patterns of 5′ promoter (a) and exon 7 (b) in caprine OPN gene.

Table 5. Genotypic frequencies and genetic polymorphism parameters at different locus of OPN gene in three goat breeds.

			Genotypic frequency			Gene frequency
		n	AA	AB	BB	A	B	Heterozygosity (He)	Polymorphism information content (PIC)	H-W (χ^2)
Dazu Black	Promoter region	60	0.767	0.233	0.000	0.883	0.117	0.207	0.185	1.040
	Exon 7	60	0.533	0.467	0.000	0.767	0.233	0.495	0.294	5.563
Lezhi Black	Promoter region	70	0.200	0.800	0.000	0.600	0.400	0.480	0.365	31.111*
	Exon 7	70	0.243	0.757	0.000	0.621	0.379	0.471	0.360	25.952*
Hexi Cashmere	Promoter region	51	0.667	0.333	0.000	0.833	0.167	0.193	0.240	1.167
	Exon 7	51	0.608	0.392	0.000	0.804	0.196	0.442	0.266	4.210

*P < 0.01.

Figure 2. Alignment of AA and AB at the site of the of 5′ promoter (a) and exon 7 (b) of OPN gene in Dazu Black goat.

Table 6. Least squares means and standard error of PE and reproduction traits at different locus of OPN gene in Dazu Black goat.

Locus	Genotype	Number of does	Litter size	Placental efficiency
5′ promoter	AA	46	2.04 ± 0.93	9.60 ± 1.88
	AB	14	1.71 ± 0.49	8.94 ± 2.11
Exon7	AA	32	2.38 ± 0.96^a	9.74 ± 2.20
	AB	28	1.50 ± 0.70^b	9.10 ± 1.43

Note: Least squares means with the different superscripts for the same locus differ significantly (P < 0.05).