Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Figures & data

Table 1. Matrix arrangement of treatments and semen samples used to test semen extenders and freezing curves on deer semen.

	Replicas
Treatment	(Ejaculate/deer)
BilSh	EJ1D1	EJ1D2	EJ1D3
BilDe	EJ1D1	EJ1D2	EJ1D3
TriSh	EJ2D1	EJ2D2	EJ2D3
TriDe	EJ2D1	EJ2D2	EJ2D3
BioSh	EJ3D1	EJ3D2	EJ3D3
BioDe	EJ3D1	EJ3D2	EJ3D3

BilSh, Biladyl^® under sheep curve; BilDe, Biladyl under deer curve; TriSh, Triladyl^® under sheep curve; TriDe, Triladyl^® under deer curve; BioSh, Bioxcell^® under sheep curve; BioDe, Bioxcell^® under deer curve. EJ_(n), ejaculated number; D_(n), deer number.

Table 2. Estimation of the semen quality index of deer (SQID) before freezing, and at the time of thawing.

Extensor/freezing curve (Treatment)	Replica	SQID % Before freezing	SQID % At the time of thawing
Triladyl^®/sheep	Deer 1	49.51	47.10
	Deer 2	61.74	58.17
	Deer 3	52.81	9.39
Triladyl^®/Deer	Deer 1	49.51	39.37
	Deer 2	61.74	55.27
	Deer 3	15.04	12.00
Bioxcell^®/Sheep	Deer 1	66.02	52.25
	Deer 2	56.21	44.62
	Deer 3	59.98	12.70
Bioxcell^®/Deer	Deer 1	66.02	59.39
	Deer 2	56.21	47.25
	Deer 3	60.16	21.97
Biladyl^®/Sheep	Deer 1	59.06	32.97
	Deer 2	10.37	7.09
	Deer 3	15.30	12.87
Biladyl^®/Deer	Deer 1	59.06	56.73
	Deer 2	32.44	23.05
	Deer 3	21.80	13.41

Figure 1. Clustering dendrogram of the trend of diluents employed in cryopreservation of deer semen, at the time of dilution, before freezing.

Table 3. Effect of semen extender before and after freezing, and effect of freezing curve at the time of thawing on the percentage of semen quality index of deer (SQID).

Source of variation	Mean (SQID %)	SD	Tukey test (α < 0.05)
Extender effect
Before freezing (p = 0301)
Bioxcell^®	60.77	±4.41	A
Triladyl^®	48.39	±17.26	AB
Biladyl^®	33.01	±21.49	B
At the time of thawing (p = 0.3684)
Bioxcell^®	39.70	±18.27
Triladyl^®	36.88	±21.33
Biladyl^®	24.36	±18.29
Freezing curve effect (p = 0.5502)
Sheep/goat	30.80	±20.43
Deer	36.50	±19.15

The Tukey test (A, B) was at the significance level of alpha of 0.05.

Figure 2. Dendrogram showing the grouping trend of freezing curves used on sheep-goat and deer semen, at the time of thawing.