The effects of acute boric acid treatment on gill, kidney and muscle tissues in juvenile rainbow trout

Figures & data

Figure 1. Normal architecture of gill sections from control (a) and histopathological changes in experimental groups. Clumping and thickening of lamellas because of inflammation (arrow in b). Lamellary edema and celluary infiltrations and clumping (arrows in e). HE.

Figure 2. Normal architecture of kidney section from the control group (a). Glomerular edema and hyperemia (arrow in b). Hyaline casts (black arrows in c and d) and glomerulitis (white arrow in c). Interstitial fibrosis around the tubule (white arrow in d) HE.

Table 1. Time- and dose-dependant comparison of histopathology in gill tissue.

	Histopathological changes in gill tissue
Groups	Hours	Lamellar oedema	Degenerative changes	Lamellary disorganization and clumping	Cellulary infiltration	Lamellar thickening
Control	All hours	–
Group II 10^2 mg/L	6	+++	–	–	–	–
	12	++	+	–	–	–
	24	++	++	–	+	–
	48	+	+++	+	++	+
	96	–	+++	+	++	+
Group III 10^3 mg/L	6	+++	–	–	–	–
	12	++	+	–	+	+
	24	++	++	++	++	++
	48	+	+++	++	+++	++
	96	–	+++	++	+++	++

Table 2. Time- and dose-dependant comparison of histopathology in kidney tissue.

	Histopathological changes in kidney tissue
Groups	Hours	Glomerular oedema	Glomerulonephritis	Degenerative changes	Hyaline casts within the tubules	Fibrous proliferation
Control	All hours	–
Group II 10^2 mg/L	6	–	–	–	–	–
	12	+	–	–	–	–
	24	++	+	+	–	–
	48	++	++	++	+	–
	96	++	++	++	++	–
Group III 10^3 mg/L	6	–	–	–	–	–
	12	+	–	–	–	–
	24	++	+	++	+	–
	48	+++	+++	+++	++	–
	96	+++	+++	+++	++	+

Table 3. Time- and dose-dependant comparison of histopathology in muscle tissue.

Groups	Hours	Interstitial oedema	Degenerative and atrophic changes in myofibrils
Control	All hours	–
Group II 10^2 mg/L	6	–	–
	12	–	–
	24	–	+
	48	+	++
	96	++	++
Group III 10^3 mg/L	6	–	–
	12	–	+
	24	+	++
	48	++	++
	96	++	+++

Figure 3. Normal architecture of musle sections from control and experimental groups (a). Intercelulary edemea (* in b), atrophic miyocytes (arrows in c). Severe edema with necrotic myocytes (black arrow in d) and myocyte losses (white arrows in d). HE.