Haematological changes in albino wister rats exposed to sub-chronic administration of sub-lethal doses of Zamitraz^® (12.5% amitraz)

Figures & data

Figure 1. The PCV values of rats given oral sub-chronic amitraz administration (range = 33–55%). Group A treated with amitraz at the dosage of 10.0 mg/kg body weight. Group B treated with amitraz at the dosage of 2.0 mg/kg body weight. Group C treated with amitraz at the dosage of 0.4 mg/kg body weight. Group D treated with water at the dosage of 10.0 mL/kg. Treatment was done daily using the oral route and a total of four different observations were made.

Figure 2. The haemoglobin values of rats given oral sub-chronic amitraz administration (range = 10.7–16 mg/dL). Group A treated with amitraz at the dosage of 10.0 mg/kg body weight. Group B treated with amitraz at the dosage of 2.0 mg/kg body weight. Group C treated with amitraz at the dosage of 0.4 mg/kg body weight. Group D treated with water at the dosage of 10.0 mL/kg. Treatment was done daily using the oral route and a total of four different observations were made.

Figure 3. The RBC values of rats given oral sub-chronic amitraz administration (range = 8.86–11.3 × 10⁶ per mL of blood). Group A treated with amitraz at the dosage of 10.0 mg/kg body weight. Group B treated with amitraz at the dosage of 2.0 mg/kg body weight. Group C treated with amitraz at the dosage of 0.4 mg/kg body weight. Group D treated with water at the dosage of 10.0 mL/kg. Treatment was done daily using the oral route and a total of four different observations were made.

Figure 4. The RTC of rats given oral sub-chronic amitraz administration (range = 0–2% of RBC). Group A treated with amitraz at the dosage of 10.0 mg/kg body weight. Group B treated with amitraz at the dosage of 2.0 mg/kg body weight. Group C treated with amitraz at the dosage of 0.4 mg/kg body weight. Group D treated with water at the dosage of 10.0 mL/kg. Treatment was done daily using the oral route and a total of four different observations were made.

Table 1. Total leucocytes and lymphocytes counts of rats treated with amitraz daily for 84 days.

Days post administration	Mean total leucocytes counts ± SEM × 10^3/mL of blood
	Group A	Group B	Group C	Group D
0	14.43	14.43	13.84	14.33
	(0.75)	(0.31)	(1.04)	(0.48)
28	9.94a	10.39a	13.42b	14.20b
	(0.44)	(0.35)	(0.28)	(0.34)
56	9.68a	9.76a	13.42b	13.53b
	(0.41)	(0.56)	(0.28)	(0.56)
84	7.19a	9.42b	11.76c	13.90d
	(0.52)	(0.49)	(0.28)	(0.59)
Mean lymphocyte counts ± SEM × 10^3/mL of blood
0	8.85	9.01	9.18	14.33
	(0.66)	(0.55)	(0.)	(0.48)
28	6.06a	6.46a	8.23b	8.83b
	(0.33)	(0.25)	(0.54)	(0.83)
56	6.77a	7.01a	8.48ab	9.17b
	(0.54)	(0.69)	(0.31)	(0.45)
84	5.30a	6.36ab	7.72b	9.56c
	(0.07)	(0.95)	(0.31)	(0.24)

Note: abc – different superscripts represents significant different at p ≤ .05. SEM, standard error of mean.

Groups A, B and C were treated with amitraz at the dosage of 10.0, 2.0 and 0.4 mg/kg body weight while group D was treated with water at the dosage of 10.0 mL/kg.

Table 2. The neutrophils and basophils counts of rats treated with amitraz daily for 84 days.

Days post administration	Mean neutrophils counts ± SEM × 10^3/mL of blood
	Group A		Group A
0	2.47	2.47	2.39	2.54
	(0.13)	(0.10)	(0.12)	(0.16)
28	1.77a	1.96ab	2.30ab	2.65b
	(0.34)	(0.10)	(0.18)	(0.18)
56	1.24a	1.34a	1.56a	2.22b
	(0.05)	(0.04)	(0.13)	(0.23)
84	0.86a	1.17a	1.75b	1.85b
	(0.04)	(0.15)	(0.08)	(0.15)
Mean basophils counts ± SEM per mL of blood
0	121.00	102.33	115.33	120.00
	(8.02)	(7.80)	(6.36)	(15.28)
28	199.67	107.67	128.00	138.00
	(59.74)	(4.18)	(9.24)	(4.16)
56	272.67a	132.67b	135.67b	137.00b
	(46.15)	(21.74)	(4.63)	(8.02)
84	219.00a	121.67b	113.33b	134.00b
	(5.86)	(19.22)	(0.88)	(6.00)

Notes: ab – different superscripts represents significant different at p ≤ .05. SEM, standard error of mean.

Groups A, B and C were treated with amitraz at the dosage of 10.0, 2.0 and 0.4 mg/kg body weight while group D was treated with water at the dosage of 10.0 mL/kg.