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Journal of Applied Animal Research Volume 45, 2017 - Issue 1
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Short Communication

Detection of BHV-1 and BRSV viruses in European bison in the Białowieża forest: a preliminary study

Renata Urban-ChmielSub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, Lublin, PolandCorrespondence[email protected]
,
Andrzej WernickiSub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, Lublin, Poland
,
Diana StęgierskaSub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, Lublin, Poland
,
Jan MarczukSub-Department of Internal Diseases of Farm Animals and Horses, Department and Clinic of Animal Internal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, Lublin, Poland
,
Jerzy RolaDepartment of Virology, National Veterinary Research Institute, Al. Partyzantów 57, Pulawy, Poland
,
Wojciech SochaDepartment of Virology, National Veterinary Research Institute, Al. Partyzantów 57, Pulawy, Poland
&
Jose L. Valverde PiedraSub-department of Toxicology and Environmental Protection, Department of Preclinical Veterinary Sciences, University of Life Sciences, Akademicka 12, Lublin, Poland
 show all
Pages 170-172 | Received 18 Mar 2015, Accepted 23 Oct 2015, Published online: 02 Feb 2016

Figures & data

Figure 1. Detection of BRSV by the RT-PCR assay in nasal swabs.

Note: M – Mol Weight standard (100–1000 bp Blirt, PL), line 1 – positive control, reference strain BRSV ATCC 375 VR1339, lines 2–4 – positive samples tested.
Figure 1. Detection of BRSV by the RT-PCR assay in nasal swabs.

Figure 2. Detection of BHV-1 in the nasal swabs by the PCR assay.

Note: M – pUC mix marker 8 mol weight standard (67–1118 bp, Fermentas, Lithuania); 1, 3–7 – BHV-1 positive samples; 2 – BHV-1 negative sample; 8 – reference strain of BHV-1, IPV468.
Figure 2. Detection of BHV-1 in the nasal swabs by the PCR assay.

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