Effect of increased incubation temperature on Hsp 90 and 60 gene expressions in coloured broiler chickens

Figures & data

Table 1. Primer sequences used for amplification of gene fragments.

Gene	Primer sequence (5′ … 3′)	Product size (bp)	Accession number
Hsp-90 alpha	F-GCATTCTCAGTTCATTGGCTACC	122	NM_206959.1
	R-CTGTCTTCTCCTCCTTCTCCTCT
Hsp-90 beta	F-CAACGATGACGAGCAGTA	127	X83230.1
	R-TCTGTCTGGTCCTCCTTC
Hsp-60	F-AGAAGAAGGACAGAGTTACC	116	NM_001012916.1
	R-GCGTCTAATGCTGGAATG
GAPDH	F-CTGCCGTCCTCTCTGGC	105	NC_006088
	R-GACAGTGCCCTTGAAGTGT

Table 2. Hsp-90 alpha m-RNA expression in heat-exposed and normal broiler chickens.

Tissues	Naked neck				PB-2
	Day old		28th day		Day old		28th day
	Heat	Normal	Heat	Normal	Heat	Normal	Heat	Normal
Heart	41.74 ± 1.06	42.55 ± 0.29	40.47 ± 0.80	39.11 ± 1.56	42.10 ± 1.71	40.26 ± 0.84	45.65 ± 0.86^a	40.11 ± 2.02^b
Liver	39.94 ± 0.51	40.83 ± 0.80	41.04 ± 0.75	39.06 ± 3.6	40.14 ± 1.27	43.64 ± 0.44	32.91 ± 0.29^Db	44.65 ± 0.44^a
Muscle	44.81 ± 0.75	43.89 ± 0.65	38.93 ± 0.63	38.66 ± 0.60	41.05 ± 0.53	41.87 ± 0.67	39.60 ± 0.81	41.89 ± 1.84
Spleen	48.46 ± 1.20	51.04 ± 1.22	38.13 ± 3.02	42.02 ± 1.70	45.85 ± 0.59^b	50.18 ± 1.52^a	35.98 ± 1.05^b	40.80 ± 0.35^a
Bursa	49.42 ± 0.64	47.55 ± 0.58	41.35 ± 1.49	46.00 ± 0.17	45.24 ± 0.58	45.83 ± 0.77	46.02 ± 0.93	48.33 ± 0.60

Note: Means with different superscripts in a row at specific age differ significantly (P < .05).

Table 3. Hsp-60 mRNA expression in heat-exposed and normal broiler chickens.

Tissues	Naked neck				PB-2
	Day old		28th day		Day old		28th day
	Heat	Normal	Heat	Normal	Heat	Normal	Heat	Normal
Heart	38.49 ± 0.90	40.96 ± 1.50	38.56 ± 0.62	40.46 ± 0.84	39.08 ± 0.71	36.96 ± 1.17	41.75 ± 1.12^a	38.50 ±  0.89^b
Liver	36.37 ± 2.22	37.83 ± 0.75	38.55 ± 0.63	40.13 ± 2.07	38.06 ± 1.39	40.33 ± 1.49	30.55 ± 1.18^b	40.78 ± 0.48^a
Muscle	40.24 ± 0.73	41.21 ± 0.76	37.32 ± 0.39	36.23 ± 1.00	36.70 ± 0.55	37.64 ± 0.69	36.65 ± 0.79	37.78 ± 0.88
Spleen	48.25 ± 0.62	47.26 ± 1.27	36.34 ± 1.47	38.67 ± 2.64	44.6 ± 1.09	44.80 ± 0.48	32.70 ± 0.19^b	35.43 ± 1.16^a
Bursa	44.75 ± 1.08	42.73 ± 0.55	40.25 ± 2.14	40.32 ± 0.16	40.06 ± 1.76	37.13 ± 0.74	41.88 ± 0.52	43.19 ± 0.18

Note: Means with different superscripts in a row at specific age differ significantly (P < .05).

Figure 1. Expression of Hsp genes in various tissues in NN chicken.

Figure 2. Expression of Hsp genes in various tissues in PB-2 chicken.