Evaluation of physiological parameters of the plasma oxidative status in rabbits

Figures & data

Table 1. Ingredients of the diet (%) and chemical composition of the diet.

Ingredients	% of Inclusion
Corn	21.2
Barley	18.5
Dehydrated alfalfa meal	50.0
Soybean seed meal	6.6
Oil	1.0
Lignosuphfite	1.5
Vitamin–mineral premix^a	1.2
Chemical composition of the diet
Dry matter, %	90.6
Organic matter, %	90.1
Crude protein, %	17.0
Crude fibre, %	18.8
Ether extract, %	4.1
Crude ash, %	9.9
Nitrogen-free extract, %	50.2
Neutral detergent fibre, %	30.5
Acid detergent fibre, %	21.4
Acid detergent lignin, %	3.9
Gross energy, MJ/kg DM	18.2

^aComposition of the Vitamin–mineral premix (per kg of diet): Vit. A 200 IU; α-tocopheryl acetate 16 mg; Niacine 72 mg; Vit. B6 16 mg; Choline 0.48 mg; DL-methionine 600 mg; Ca 500 mg; P 920 mg; K 500 mg; Na 1 g; Mg 60 mg; Mn 1.7 mg; Cu 0.6 mg.

Table 2. Productive performance (means ± SEM) of rabbits.

Initial weight, g	2318 ± 7.3
Final weight, g	3562 ± 10.9
Total feed consumption, g	7936 ± 37.4
Total weight gain, g	1240 ± 12.7
Feed/gain ratio, g/g	6.38 ± 0.04
Mortality, %	0

Table 3. Biochemical analysis of rabbits’ serum.

Biochemical parameters	Initial values (mean ± sem)	Final values (mean ± sem)
Albumin, g/dl	3.997 ± 0.029	4.007 ± 0.029
Triglycerides, mg/dl	128.1 ± 1.681	128.033 ± 1.866

Table 4. Values of reactive oxygen metabolites (d-ROMs test, CARR U) and antioxidant activity (fast antioxidants and slow antioxidants, anti-ROMs test, µEq of reduced iron/litre) in rabbit serum at the beginning and at the end of the experimental period.

Biochemical parameters	Initial values (mean ± sem)	Final values (mean ± sem)
Reactive oxygen metabolites (d-ROMs test)	299.9 ± 6.1	296.8 ± 6.4
Fast antioxidants (anti-ROMs test)	85.2 ± 2.1	85.4 ± 2.7
Slow antioxidants (anti-ROMs test)	594.7 ± 4.2	597.2 ± 4.7

Table 5. Confidence intervals for the studied parameters.

Parameters	(CI) lower values	(CI) upper values
Reactive oxygen metabolites (d-ROMs test, CARR U)	283.991	309.543
Fast antioxidants (anti-ROMs test, µEq of reduced iron/litre)	80.037	90.762
Slow antioxidants (anti-ROMs test, µEq of reduced iron/litre)	590.122	604.211
Albumin, g/dl	3.964	4.066
Triglycerides, mg/dl	124.319	131.747